Comparison of QTLs for rice seedling morphology under different water supply conditions

Bingsong Zheng; Ling Yang; Chuanzao Mao; Youjun Huang; Ping Wu

doi:10.1016/S1673-8527(08)60065-X

Volume 35 Issue 8

Aug. 2008

Turn off MathJax

Article Contents

Article Navigation > Journal of Genetics and Genomics > 2008 > 35(8): 473-484

PDF( 610 KB)

Comparison of QTLs for rice seedling morphology under different water supply conditions

doi: 10.1016/S1673-8527(08)60065-X

a
School of Forestry & Biotechnology, Zhejiang Forestry University, Hangzhou 311300, China
b
State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou 310058, China
c
College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua 321004, China

More Information

Corresponding author: E-mail address: bszheng@zjfc.edu.cn (Bingsong Zheng)
Received Date: 2008-02-17
Accepted Date: 2008-05-16
Rev Recd Date: 2008-05-12

Available Online: 2008-08-20

Publish Date: 2008-08-20

Abstract

Abstract

The variation of seedling characteristics under different water supply conditions is strongly associated with drought resistance in rice (Oryza sativa L.) and a better elucidation of its genetics is helpful for improving rice drought resistance. Ninetysix doubled-haploid (DH) rice lines of an indica and japonica cross were grown in both flooding and upland conditions and QTLs for morphological traits at seedling stage were examined using 208 restriction fragment length polymorphism (RFLP) and 76 microsatellite (SSR) markers. A total of 32 putative QTLs were associated with the four seedling traits: average of three adventitious root lengths (ARL), shoot height (SH), shoot biomass (SW), and root to shoot dry weight ratio (RSR). Five QTLs detected were the same under control and upland conditions. The ratio between the mean value of the seedling trait under upland and flooding conditions was used for assessing drought tolerance. A total of six QTLs for drought tolerance were detected. Comparative analysis was performed for the QTLs detected in this case and those reported from two other populations with the same upland rice variety Azucena as parent. Several identical QTLs for seedling elongation across the three populations with the positive alleles from the upland rice Azucena were detected, which suggests that the alleles of Azucena might be involved in water stress-accelerated elongation of rice under different genetic backgrounds. Five cell wall-related candidate genes forOsEXP1, OsEXP2, OsEXP4, EXT, and EGase were mapped on the intervals carrying the QTLs for seedling traits.
- QTLs mapping,
- seedling morphology,
- flooding condition,
- upland condition

FullText(HTML)

References(27)

References

[1]	Champoux, M.C., Wang, G., Sarkarung, S. et al. Theor. Appl. Genet., 90 (1995),pp. 969-981
[2]	Cho, Y.G., McCouch, S.R., Kuiper, M. et al. Theor. Appl. Genet., 97 (1998),pp. 370-380
[3]	Cosgrove, D.J. Enzymes and other agents that enhance cell wall extensibility Annu. Rev. Plant Physiol. Plant Mol. Biol., 50 (1999),pp. 391-417
[4]	Courtois, B., McLaren, G., Sinha, P.K. et al. Mapping QTLs associated with drought avoidance in upland rice Mol. Breed., 6 (2000),pp. 55-66
[5]	Darvasi, A., Soller, M. A simple method to calculate resolving power and confidence interval of QTL map location Behav. Genet., 27 (1997),pp. 125-132
[6]	Garrity, D.P., Oldeman, L.R., Morris, R.A.
[7]	Harushima, Y., Yano, M., Shomura, A. et al. Genetics, 148 (1998),pp. 479-494
[8]	Hemamalini, G.S., Shashidhar, H.E., Hittalmani, S. Molecular marker assisted tagging of morphological and physiological traits under two contrasting moisture regimes at peak vegetative stage in rice Euphytica, 112 (2000),pp. 69-78
[9]	Hittalmani, S., Huang, N., Courtois, B. et al. Identification of QTL for growth- and grain yield-related traits in rice across nine location of Asia Theor. Appl. Genet., 107 (2003),pp. 679-690
[10]	Huang, N., Courtois, B., Khush, G.S. et al. Association of quantitative traits loci for plant height with major dwarfism genes in rice Heredity, 77 (1996),pp. 130-137
[11]	Kamoshita, A., Wade, L.J., Ali, M.L. et al. Mapping QTLs for root morphology of a rice population adapted to rainfed lowland conditions Theor. Appl. Genet., 104 (2002),pp. 880-893
[12]	Li, Z.K., Yu, S.B., Lafitte, H.R. et al. QTL × environment interactions in rice. I. Heading date and plant height Theor. Appl. Genet., 108 (2003),pp. 141-153
[13]	Liao, C.Y., Wu, P., Hu, B. et al. Theor. Appl. Genet., 103 (2001),pp. 104-111
[14]	MacMillan, K., Emrich, K., Piepho, H.-P. et al. Assessing the importance of genetype × environment interaction for root traits in rice using a mapping population II: Conventional QTL analysis Theor. Appl. Genet., 113 (2006),pp. 953-964
[15]	Nelson, J.C. QGENE: Software for marker-based genomic analysis and breeding Mol. Breed., 3 (1997),pp. 239-245
[16]	Price, A.H., Steele, K.A., Moore, B.J. et al. Upland rice grown in soil-filled chambers and exposed to contrasting water-deficit regimes: II. Mapping QTL for root morphology and distribution Field Crops Res., 76 (2002),pp. 25-43
[17]	Price, A.H., Tomos, A.D. Theor. Appl. Genet., 95 (1997),pp. 143-152
[18]	Spielmeyer, W., Ellis, M.H., Chandler, P.M. Semidwarf (sd-1), “green revolution” rice, contains a defective gibberellin 20-oxidase gene Proc. Natl. Acad. Sci. USA, 99 (2002),pp. 9043-9048
[19]	Venuprasad, R., Shashidhar, H.E., Hittalmani, S. et al. Euphytica, 128 (2002),pp. 293-300
[20]	Yadav, R., Courtois, B., Huang, N. et al. Mapping genes controlling root morphology and root distribution in a doubled-haploid population of rice Theor. Appl. Genet., 94 (1997),pp. 619-632
[21]	Yang, L., Wang, C.C., Guo, W.D. et al. Differential expression of cell wall related genes in the elongation zone of rice roots under water deficit Russ. J. Plant Physiol., 53 (2006),pp. 437-443
[22]	Yoshida, S., Forno, D.A., Cock, J.H. et al.
[23]	Zhang, W.P., Shen, X.Y., Wu, P. et al. Theor. Appl. Genet., 103 (2001),pp. 118-123
[24]	Zhang, W.P., Wu, P., Shen, X.Y. et al. Genetic analysis of root growth in rice seedlings under different water supply condition Acta Bot. Sin., 43 (2001),pp. 1024-1030
[25]	Zheng, H.G., Babu, R.C., Pathan, M.S. et al. Quantitative trait loci for root penetration ability and root thickness in rice: Comparison of genetic backgrounds Genome, 43 (2000),pp. 53-61
[26]	Zheng, B.S., Yang, L., Zhang, W.P. et al. Comparison of QTLs for rice root morphology under different water supply conditions Acta Genetica Sinica, 33 (2006),pp. 141-151
[27]	Zheng, B.S., Yang, L., Zhang, W.P. et al. Mapping QTLs and candidate genes for rice root traits under different water-supply conditions and comparative analysis across three populations Theor. Appl. Genet., 107 (2003),pp. 1505-1515