Identification and characterization of cDNA sequences encoding the HIS3 and LEU2 genes of the fungus Alternaria tenuissima

Ying Wan; Xuli Wang; Yun Huang; Dewen Qiu; Linghuo Jiang

doi:10.1016/S1673-8527(08)60035-1

Volume 35 Issue 4

Apr. 2008

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Article Navigation > Journal of Genetics and Genomics > 2008 > 35(4): 251-256

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Identification and characterization of cDNA sequences encoding the HIS3 and LEU2 genes of the fungus Alternaria tenuissima

doi: 10.1016/S1673-8527(08)60035-1

a
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100094, China
b
Department of Plant Protection, College of Agricultural Sciences, Sichuan Agricultural University, Ya'an 625014, China

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Corresponding author: E-mail address: linghuojiang2@yahoo.com.cn (Linghuo Jiang)
Received Date: 2007-09-20
Accepted Date: 2007-12-10
Rev Recd Date: 2007-12-05

Available Online: 2008-04-24

Publish Date: 2008-04-20

Abstract

Abstract

Alternaria tenuissima is a fungus widely present in the environment and could cause diseases in plants and humans. In this study, through a yeast genetic approach, cDNA sequences were isolated and characterized for the AtHIS3 and AtLEU2 genes. AtHIS3 cDNA encodes a protein of 238 amino acids, while AtLEU2 cDNA encodes a protein of 363 amino acids. Based on the phylogenetic analysis of amino acid sequences of AtHis3p and AtLeu2p, A. tenuissima is closely related to the plant pathogenic fungus Phaeosphaeria nodorum. This study provides two genetic markers for studies of functions of genes regulating development, morphology, and virulence ofA. tenuissima.
- Alternaria tenuissima,
- AtHIS3,
- AtLEU2,
- cDNA,
- genetic marker,
- pathogen

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References(38)

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