Fine Mapping and Isolation of Bc7(t), Allelic to OsCesA4

Changjie Yan; Song Yan; Xiuhong Zeng; Zhengqiu Zhang; Minghong Gu

doi:10.1016/S1673-8527(07)60115-5

Volume 34 Issue 11

Nov. 2007

Turn off MathJax

Article Contents

Abstract

References

Article Navigation > Journal of Genetics and Genomics > 2007 > 34(11): 1019-1027

PDF( 542 KB)

Fine Mapping and Isolation of Bc7(t), Allelic to OsCesA4

doi: 10.1016/S1673-8527(07)60115-5

a
Key Laboratory of Plant Functional Genomics, Ministry of Education, PR of China/Jiangsu Key Laboratory for Crop Genetics and Physiology, Yangzhou University, Yangzhou 225009, China
b
Bureau of Agriculture of Lianyungang, Lianyungang 222000, China

More Information

Corresponding author: E-mail address: yichuan@yzu.edu.cn (Minghong Gu)
Received Date: 2007-06-18
Accepted Date: 2007-07-28

Available Online: 2007-11-22

Publish Date: 2007-11-20

Abstract

Abstract

Several brittle culm mutants of rice were identified and characterized. In this study, we characterized a brittle mutant (bc7(t)) identified from japonica variety Zhonghua 11 by means of ⁶⁰Co-γ radiation. This mutant displays normal phenotype similar to its wild type plants except for the fragility of all plant body, with ∼10% decrease in the cellulose content. The genetic analysis and gene fine mapping showed that the bc7(t) mutant was controlled by a recessive gene, residing on an 8.4 kb region of the long arm of chromosome 1. The gene annotation indicated that there was only one putative gene encoding cellulose synthase catalytic subunit (CesA) in this region, which was allelic to OsCesA4. Furthermore, the sequence analysis was carried out and 7 bases deletion in the junction of exon 10 and intron 10 was done in bc7(t) mutant, resulting in the change of reading frame and the consequent failure to generate functional protein. In addition, the result of RNA interference experiment showed that when theBc7(t) was knocked down, the transplants exhibited fragility, similar to bc7(t) mutant. The finding of novel allele of OsCesA4 locus will facilitate the understanding of the mechanism of cell wall biosynthesis. The potential utilization of the bc7(t) mutant in animal food was discussed as well.
- rice,
- brittle culm,
- gene cloning,
- RNAi

FullText(HTML)

References(25)

References

[1]	Taylor, NG, Scheible, et al. Plant Cell, 11 (1999),pp. 769-779
[2]	Kokubo, A, Kuraishi, et al. Culm strength of barley: correlation among maximum bending stress, cell wall dimensions, and cellulose content Plant Physiol, 91 (1989),pp. 876-882
[3]	Kokubo, A, Sakurai, et al. Plant Physiol, 97 (1991),pp. 509-514
[4]	Delmer, DP, Amor, et al. Cellulose biosynthesis Plant Cell, 7 (1995),pp. 987-1000
[5]	Pear, JR, Kawagoe, et al. Proc Natl Acad Sci USA, 93 (1996),pp. 12637-12642
[6]	Tanaka, K, Murata, et al. Three Distinct Rice Cellulose Synthase Catalytic Subunit Genes Required for Cellulose Synthesis in the Secondary Wall Plant Physiol, 133 (2003),pp. 73-83
[7]	Kinoshita, T Report of committee on gene symbolization, nomenclature and linkage groups Rice Genet News, 12 (1995),pp. 9-153
[8]	Li, YH, Qian, et al. Plant Cell, 15 (2003),pp. 2020-2031
[9]	Van Soest, PJ, Robertson, et al. Analysis of forage and fibrous food. A laboratory manual for animal science 613 Cornel University, Ithaca, New York (1985),pp. 74-75
[10]	Rogers, SO, Bendch, et al. Extraction of DNA from plant tissues Plant Molecular Biology Manual, 6 (1988),pp. 1-10
[11]	Michelmore, RW, Paran, et al. Identification of markers linked to disease resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions using segregating populations Proc Natl Acad Sci USA, 88 (1991),pp. 9828-9832
[12]	Lander, ES, Green, et al. MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations Genomics, 1 (1987),pp. 174-181
[13]	Hiei, Y, Ohta, et al. Plant J, 6 (1994),pp. 271-282
[14]	Jones, JW Inheritance of characters in rice J Agric Res, 47 (1933),pp. 771-782
[15]	Takahashi, R, Yamamoto, et al. Inheritance and linkage studies in barley Ber Ohara Inst landw Forsch, 10 (1953),pp. 29-52
[16]	Takahashi, R, Hayashi, et al. Inheritance and linkage studies in barley: III. Linkage studies of the gene for fragile stem-2 and orientation of linkage map on barley chromosome 5 Ber Ohara Inst landw Biol Okayama Univ, 13 (1966),pp. 199-212
[17]	Takahashi, M, Kinoshita, et al. Character expressions and casual genes of some mutants in rice plant: XXXIII. Genetical studies on rice plant J Fac Agr, Hokkaido Univ, Sapporo, 55 (1968),pp. 496-512
[18]	Nagano, S, Takahashi, et al. Trial construction of twelve linkage groups in Japanese rice: XXVII. Genetical studies on rice plant J Fac Agr, Hokkaido Univ, Sapporo, 53 (1963),pp. 72-130
[19]	Briggs, RW, Robert, et al. Recognition and classification of some genetic traits in maize J Hered, 57 (1966),pp. 35-42
[20]	Turner, SR, Somerville, et al. Collapsed xylem phenotype of Arabidopsis in the secondary cell wall Plant Cell, 9 (1997),pp. 689-701
[21]	Taylor, NG, Laurie, et al. Plant Cell, 12 (2000),pp. 2529-2539
[22]	Jones, L, Ennos, et al. Plant J, 26 (2001),pp. 205-216
[23]	Zhong, R, Burk, et al. A kinesin-like protein is essential for oriented deposition of cellulose microfibrils and cell wall strength Plant Cell, 14 (2002),pp. 3101-3117
[24]	Shen, H, Sundstol, et al. Studies on untreated and urea-treated rice straw from three cultivation seasons: Histological investigations by light and scanning electron microscopy Anim Feed Sci Tech, 80 (1999),pp. 151-159
[25]	Kobayashi, Y, Tanaka, et al. Effect of ammonia treatment on physical strength of rice straw, distribution of straw particles and particle-associated bacteria in sheep rumen Anim Feed Sci Tech, 115 (2004),pp. 117-128