Improvement for Agronomic Traits of Partial Waxy Wheat by Combination of Backcrossing with a PCR-based DNA Marker

Yuxiu Dong; Xiangyu Zhao; Jiawei Wang; Guoliang Yuan; Xiansheng Zhang

doi:10.1016/S1673-8527(07)60094-0

Volume 34 Issue 9

Sep. 2007

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Article Navigation > Journal of Genetics and Genomics > 2007 > 34(9): 836-841

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Improvement for Agronomic Traits of Partial Waxy Wheat by Combination of Backcrossing with a PCR-based DNA Marker

doi: 10.1016/S1673-8527(07)60094-0

Shandong Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai'an 271018, China

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Corresponding author: E-mail address: zhangxs@sdau.edu.cn (Xiansheng Zhang)
Received Date: 2006-11-17
Accepted Date: 2007-03-19

Available Online: 2007-09-18

Publish Date: 2007-09-20

Abstract

Abstract

To improve agronomic traits of partial waxy wheat, crossing between Chinese Baihuomai and wheat cultivars PH85-16, Jinan 17, and Yannong 15 was performed. The progeny plants were further backcrossed to these cultivars as recurrent parents for five generations. To get homozygous plants with the null allele at the Wx-D1 locus, self-pollination was carried out in the BC₅F₁ generation. Through another three generations, 6 partial waxy wheat lines were obtained, which had similar agronomic performance as their recurrent parents and carried the null allele at the Wx-D1 locus. In each generation, the Wx-D1 locus was identified by a PCR-based DNA marker and the agronomic traits were examined in progeny plants. The results from this study indicate that the use of backcrossing with a PCR-based DNA marker was useful in waxy wheat breeding. These partial waxy wheat lines can be used in field production.
- partial waxy wheat,
- improved agronomic traits,
- backcrossing,
- molecular assisted selection

These authors contributed equally to this work.

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References(22)

References

[1]	P, Sathish, C, et al. Modified starch metabolism in mutant and transgenic plants Prog Bot, 56 (1995),pp. 301-318
[2]	M, Yamamori, NT, et al. Differential effects of Wx-A1, -B1 and -D1 protein deficiencies on apparent amylose content and starch pasting properties in common wheat Theor Appl Genet, 100 (2000),pp. 32-38
[3]	T, Nakamura, M, et al. Identitfication of three Wx proteins in wheat J Biochem Genet, 31 (1993),pp. 75-86
[4]	J, Murai, T, et al. Isolation and characterization of the three waxy genes encoding the granule-bound starch synthase in hexaploid wheat Gene, 234 (1999),pp. 71-79
[5]	T, Nakamura, M, et al. Production of waxy (amylase-free) wheats Mol Gen Genet, 248 (1995),pp. 253-259
[6]	T, Nakamura, P, et al. Rapid classification of partial waxy wheats using PCR-based markers Genome, 45 (2002),pp. 1150-1156
[7]	JJ, Liu, ZH, et al. Variation of starch property and its relationship with dry white Chinese noodle quality in common wheat Agricultural Sciences in China, 2 (2003),pp. 1-7
[8]	M, Yamamori, T, et al. Waxy protein deficiency and chromosomal location of coding genes in common wheat Theor Appl Genet, 89 (1994),pp. 179-184
[9]	XC, Zhao, PJ, et al. Plant Breed, 117 (1998),pp. 488-490
[10]	P, Vrinten, T, et al. Molecular characterization of waxy mutations in wheat Mol Gen Genet, 261 (1999),pp. 463-471
[11]	MR, Shariflou, ME, et al. Plant Breed, 120 (2001),pp. 121-124
[12]	A, Briney, R, et al. A PCR-based marker for selection of starch and potential noodle quality in wheat Mol Breed, 4 (1998),pp. 427-433
[13]	MR, Shariflou, PJ, et al. Plant Breed, 118 (1999),pp. 275-277
[14]	M, Saito, M, et al. Theor Appl Genet, 108 (2004),pp. 1205-1211
[15]	AM, Monari, MC, et al. Molecular characterization of new waxy mutants identified in bread and durum wheat Theor Appl Genet, 110 (2005),pp. 1481-1489
[16]	PH, Zhou, YF, et al. Simultaneous improvement for four quality traits of Zhenshan 97, an elite parent of hybrid rice, by molecular marker-assisted selection Theor Appl Genet, 106 (2003),pp. 326-331
[17]	SG, Shu, T, et al. Developing waxy wheat with backcrossing approach and molecular marker-assisted selection Hereditas, 28 (2006),pp. 563-570
[18]	DN, Yao, XW, et al. Identification and screen of Wx protein in wheat J Agri Biotech, 7 (1999),pp. 1-9
[19]	M, Yamamori, M, et al. Euphytica, 64 (1992),pp. 215-219
[20]	XC, Zhao, PJ, et al. An improved 1-D SDS-PAGE method for the identification of three bread wheat ‘waxy’ protein J Cereal Sci, 23 (1996),pp. 191-193
[21]	FM, Ausubel, R, et al.
[22]	S, Tomoko, Y, et al. Cereal Chem, 77 (2000),pp. 58-63