Transgenic Mini-tomato and Protection Against Alcohol-induced Gastric Injury

Qingwen Zhi; Shuhao Wang; Min Chai; Fengying Zhang; Qian Li; Shigui Li; Manji Sun

doi:10.1016/S1673-8527(07)60085-X

Volume 34 Issue 8

Aug. 2007

Turn off MathJax

Article Contents

Article Navigation > Journal of Genetics and Genomics > 2007 > 34(8): 756-763

PDF( 317 KB)

Transgenic Mini-tomato and Protection Against Alcohol-induced Gastric Injury

doi: 10.1016/S1673-8527(07)60085-X

a
Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100850, China
b
Key Laboratory of Crop Genetic Resource and Improvement of Ministry of Education, Sichuan Agricultural University, Ya'an 625014, China
c
Institute of Vegetable, Beijing Academy of Agriculture and Forest, Beijing 100097, China
d
Command and Engineering College of Chemical Defense of Chinese PLA, Beijing 102205, China

More Information

Corresponding author: E-mail address: lishigui_sc@263.net (Shigui Li); E-mail address: sunmj@nic.bmi.ac.cn (Manji Sun)
Received Date: 2006-11-20
Accepted Date: 2006-12-06

Available Online: 2007-08-21

Publish Date: 2007-08-20

Abstract

Abstract

The epidermal growth factor (EGF) has been shown to promote the proliferation of various types of cells, to maintain the physiological function of the mucosa of the digestive tract, and to promote the healing of the gastric and duodenal ulcers. It has been expressed in many types of bacteria and yeasts. In this article, a bio-reactor was constructed, namely, the human EGF (hEGF) transgenic mini-tomato. On the basis of hEGF gene sequence, a tomato codon preference hEGF gene was chemically synthesized, and it was constructed into the plant expression vector pCAMBIA2300. The transgenic tomato plants containing gene hEGF were obtained through Agrobacterium-mediated transformation. The expression product was determined by radioimmunoassay (RIA) and showed a yield of 3.48 ± 1.01 ng/g fresh fruits. The intragastric gavage (ig) administration of the rhEGF–containing juice of the transgenic tomato (equivalent to 24 ng rhEGF per mouse a day) for 15 days could significantly protect mice against the alcohol-induced ulceration. The ulcer index, expressed as a degree of the stomach lesion, decreased from 42.20 ± 18.13 to 16.25 ± 9.57.
- tomato,
- expression,
- gastric ulcer

FullText(HTML)

References(18)

References

[1]	Nakagawa, S, Yoshida, et al. Differentiation, 29 (1985),pp. 284-288
[2]	Konturek, SJ, Dembinski, et al. Role of epidermal growth factor in healing of chronic gastroduodenal ulcer in rats Gastroenterology, 94 (1988),pp. 1300-1307
[3]	Konturek, PC, Brzozowski, et al. J Physiol–Paris, 95 (2001),pp. 361-367
[4]	Marti, V, Burwen, et al. Biological effects of epidermal growth factor, with emphasis on the gastrointestinal tract and liver: An update Hepatolgy, 9 (1989),pp. 126-138
[5]	Salvadori, G, Surrenti, et al. Role of epidermal growth factor in peptic ulcer healing Digest Dis Sci, 40 (1995),pp. 2497-2504
[6]	Itoh, M, Imai, et al. Protection of gastric mucosa against ethanol-induced injury by intragastric bolus administration of epidermal growth factor combined with hydroxypropylcellulose J Clin Gastroenterol, 14 (1992),pp. S127-S130
[7]	Yamagata, H, Nakahama, et al. Proc Natl Acad Sci USA, 86 (1989),pp. 3589-3593
[8]	Sivakesava, S, Xu, et al. Process Biochem, 34 (1999),pp. 893-900
[9]	Tong, WY, Yao, et al. Appl Microbiol Biotechnol, 57 (2001),pp. 674-679
[10]	Lam, KH, Chow, et al. J Biotechnol, 63 (1998),pp. 167-177
[11]	Wang, GL, Yi, et al. Acta Genetica Sinica, 30 (2003),pp. 97-102
[12]	Clements, JM, Catlin, et al. Gene, 106 (1991),pp. 267-272
[13]	Heo, JH, Won, et al. Protein Exp Purif, 24 (2002),pp. 117-122
[14]	Higo, K, Saito, et al. Expression of chemically synthesized gene for human epidermal growth factor under the control of cauliflower mosaic virus 35S promoter in transgenic tobacco Biosci Biotech Biochem, 57 (1993),pp. 1477-1481
[15]	van Santen, VL, Spritz, et al. Splicing of plant pre-mRNAs in animal systems and vice versa Gene, 56 (1987),pp. 253-265
[16]	Hunt, AG, Chu, et al. Plant cells do not properly recognize animal gene polyadenylation signals Plant Mol Biol, 8 (1987),pp. 23-35
[17]	Tunio, AM, Hobsley, et al. Epidermal growth factor in Saliva and gastric juice response to histamine Gut, 37 (1995),pp. 335-339
[18]	Calabro, A, Orsini, et al. Gastric juice immunoreactive epidermal growth factor levels in patients with peptic ulcer disease Am J Gastroenterol, 85 (1990),pp. 404-407