Analysis of Differentially Expressed Genes in Genic Male Sterility Cotton (Gossypium hirsutum L.) Using cDNA-AFLP

Xiaoding Ma; Chaozhu Xing; Liping Guo; Yangcang Gong; Hailin Wang; Yunlei Zhao; Jianyong Wu

doi:10.1016/S1673-8527(07)60059-9

Volume 34 Issue 6

Jun. 2007

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Article Navigation > Journal of Genetics and Genomics > 2007 > 34(6): 536-543

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Analysis of Differentially Expressed Genes in Genic Male Sterility Cotton (Gossypium hirsutum L.) Using cDNA-AFLP

doi: 10.1016/S1673-8527(07)60059-9

a
Cotton Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory for Cotton Genetic Improvement, Ministry of Agriculture, Anyang 455000, China
b
Huazhong Agricultural University, Wuhan 430070, China

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Corresponding author: E-mail address: xingcz@cricaas.com.cn (Chaozhu Xing)
Received Date: 2006-11-15
Accepted Date: 2006-12-07

Available Online: 2007-06-27

Publish Date: 2007-06-20

Abstract

Abstract

cDNA amplified fragment length polymorphism (cDNA-AFLP) analysis was used to investigate the differentially expressed genes between sterile and fertile plants of ms5ms6 double-recessive genic male sterility (GMS) two-type line cotton (Gossypium hirsutum L.) at different stages, i.e., sporogenous cell stage, pollen mother cell (PMC) stage, and pollen grain stage. Seventeen differentially expressed fragments were identified. Functional analysis indicated that their corresponding genes may participate in the processes of signal transduction, transcription, energy metabolism, and plant cell wall development. Northern blot demonstrated the credibility of the result of cDNA-AFLP. A sterility restorer factor-like gene, which only expressed in fertile anther and was notably homologous to T cytoplasm male sterility restorer factor 2 of maize (Zea mays L.), was identified in this research.
- cotton,
- cDNA-AFLP,
- gene expression,
- double-recessive GMS

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References(23)

References

[1]	Xing, CZ, Guo, et al. Study on heterosis and insert pollination of nuclear sterile of upland cotton resistant to cotton boll worm-Zhongkang A. Acta Agronomica Sinica, 28 (2002),pp. 574-576
[2]	Hou, L, Xiao, et al. The cDNA-AFLP differential display in developing anthers between cotton male sterile and fertile line of “Dong A”. Acta Genetica Sinica, 29 (2002),pp. 359-363
[3]	Bachem, CWB, van der Hoeven, et al. Visualization of differential gene expression using a novel method of RNA fingerprinting based on AFLP: analysis of gene expression during potato tuber de velopment Plant J., 9 (1996),pp. 745-753
[4]	Bachem, CWB, Oomen, et al. Transcript imaging with cDNA-AFLP: A step-by-step protocol Plant Mol Biol Rep., 16 (1998),pp. 157-173
[5]	Song, GL, Zhang, et al. Cotton AFLP analysis with silver-staining and preminary report of variety fingerprinting based on it Acta Gossypii Sinica, 11 (1999),pp. 281-283
[6]	Li, ZY, Chen, et al. Rapid screening and classification of positive cDNA clones generated by differential display High Technology Letters, 8 (1999),pp. 44-48
[7]	Vos, P, Hogers, et al. AFLP: a new technique for DNA finger printing Nucleic Acids Res., 23 (1995),pp. 4407-4414
[8]	Sambrook, J, Fritsh, et al.
[9]	Money, T, Reader, et al. AFLP-based mRNA fingerprinting Nucleic Acids Res., 24 (1996),pp. 2616-2617
[10]	Xiao, YH, Luo, et al. cDNA-AFLP analysis of the gene expression in cotton ovule at fiber initiation stage Journal of Agricultural Biotechnology, 11 (2003),pp. 20-24
[11]	Wu, MS, Gao, et al. Studies on differential gene expression of maize (Zea mays L.) by means of cDNA-AFLP technique Acta Agronimica Sinica, 27 (2001),pp. 339-342
[12]	Wu, JY, Shen, et al. Differentially distinguished expressed genes in dominant genic male sterility (DGMS) Brassica napususing cDNA-AFLP Scientia Agricultura Sinica, 39 (2006),pp. 1921-1926
[13]	McCormick, S Control of male gametophyte development Plant Cell, 16 (2004),pp. S142-S153
[14]	Ma, XD, Xing, et al. Advanced study and utilization of the male sterility in cotton Acta Gossypii Sinica, 18 (2006),pp. 309-314
[15]	Yule, Liu, Burch-Smith, et al. Molecular chaperone Hsp90 associates with resistance protein N and Its signaling proteins SGT1 and Rar1 to modulate an innate immuneresponse in Plants J Biol Chem., 279 (2004),pp. 2101-2108
[16]	Tenhaken, R, Thulke, et al. Cloning of an enzyme that synthesizes a key nucleotide sugar precursor of hemicellulose biosynthesis from soybean: UDP-Glucose dehydrogenase Plant Physiol., 112 (1996),pp. 1127-1134
[17]	Cigan, AM, Unger, et al. Phenotypic complementation of ms45 maize requires tapetal expression of MS45 Sex Plant Rerord., 14 (2001),pp. 135-142
[18]	Albertsen, MC, Philips, et al. Developmental cytology of 13 genetic male sterile loci in maize Can J Genet Cytol., 23 (1981),pp. 195-208
[19]	Gorman, SW, McCormick, et al. Male sterility in tomato Crit Rev Plant Sci., 16 (1997),pp. 31-53
[20]	Liu, F, Cui, et al. Mitochondrial aldehyde dehydrogenase activity is required for male fertility in Maize Plant Cell, 13 (2001),pp. 1063-1078
[21]	Kennell, JC, Pring, et al. Initiation and processing of atp6, T-urf13 and ORF221 transcripts from mitochondria of T-cytoplasm maize Mol Gen Genet., 216 (1989),pp. 16-24
[22]	Wise, RP, Bronson, et al. The genetics, pathology and molecular biology of T-cytoplasm male sterility in maize Adv Agron., 65 (1999),pp. 79-130
[23]	Dewey, RE, Timothy, et al. A mitochondrial protein associated with cytoplasmic male sterility in the T cytoplasm of maize Proc Natl Acad Sci USA, 84 (1987),pp. 5374-5378