Identification of Prosaposin as a Novel Interaction Partner for Rhox5

Fen Guo; Xiaofeng Huang; Shiqian Li; Limin Sun; Yueqin Li; Hongjian Li; Yi Zhou; Yanhui Chu; Tianhong Zhou

doi:10.1016/S1673-8527(07)60042-3

Volume 34 Issue 5

May 2007

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Article Navigation > Journal of Genetics and Genomics > 2007 > 34(5): 392-399

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Identification of Prosaposin as a Novel Interaction Partner for Rhox5

doi: 10.1016/S1673-8527(07)60042-3

a
College of Life Science and Technology, Jinan University, Guangzhou 510632, China
b
Mu Dan Jiang Medical University, Heilongjiang 157011, China

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Corresponding author: E-mail address: tzth@jnu.edu.cn (Tianhong Zhou)
Received Date: 2006-10-25
Accepted Date: 2006-11-29

Available Online: 2007-06-07

Publish Date: 2007-05-20

Abstract

Abstract

Prosaposin (Psap) has multiple cellular functions. It is involved in the development of the reproductive system, nervous system, and prostate cancer as well as in the regulation of sphingolipid catabolism by activating several lysosomal hydrolases involved in the metabolism of various sphingolipids. In this research, it was found to be a novel interaction partner for Rhox5 using yeast two-hybrid screening. The interaction between Rhox5 and the full-length prosapsoin (the transcript without exon 8) as well as the C-terminal domain of prosaposin, was further confirmed in both yeast two hybrid analysis and in vitro assay. It suggested that the C-terminal domain of prosaposin may be critical for the Rhox5-prosaposin interaction. Given the important roles played by both Rhox5 and prosaposin in maintaining the differentiation of male reproductive organs, spermatogenesis, and fertilization, the interaction between Rhox5 and prosaposin might regulate the development of male reproductive organs dynamically.
- Rhox5,
- prosaposin,
- functional interaction,
- reproductive system

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References(25)

References

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