Comparative Microsatellite Analysis of Grass Carp Genomes of Two Gynogenetic Groups and the Xiangjiang River Group

Kang Zheng; Kaidong Lin; Zhenghua Liu; Chen Luo

doi:10.1016/S1673-8527(07)60034-4

Volume 34 Issue 4

Apr. 2007

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Article Navigation > Journal of Genetics and Genomics > 2007 > 34(4): 321-330

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Comparative Microsatellite Analysis of Grass Carp Genomes of Two Gynogenetic Groups and the Xiangjiang River Group

doi: 10.1016/S1673-8527(07)60034-4

Institute of Biology, Hunan Normal University, Changsha 410081, China

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Corresponding author: E-mail address: cluo6009@163.com (Chen Luo)
Received Date: 2006-07-25
Accepted Date: 2006-08-24

Available Online: 2007-05-09

Publish Date: 2007-04-20

Abstract

Abstract

The genomes of three groups of grass carp, namely the Xiangjiang River grass carp group (Xiangjiang group), a one-generation artificially induced meio-gynogenetic grass carp group (meio-gynogenetic-1 group), and a two-generation artificially induced meio-gynogenetic grass carp group (meio-gynogenetic-2 group), were comparatively analyzed with microsatellite markers. Genetic polymorphism had been observed in the Xiangjiang group and most of the examined loci had more than two alleles. But the degree of genetic diversity was not very high. Although all the examined genetic loci in the analyzed individuals were in homozygous state, the genotypes of different individuals of the group were not identical in the meio-gynogenetic-1 group. In the meio-gynogenetic-2 group, not only the examined genetic loci of each individual were homozygous but also the genotypes of all the analyzed individuals of the group were the same. These results suggested that the examined meio-gynogenetic-2 group is a homozygous group and homozygous clone could be produced by continuous artificial induction of gynogenesis for two generations. It was found that the polymorphism existed not only at the allele level but also at the locus level; many alleles of the microsatellite loci and some of the microsatellite loci had been lost during the process of artificial gynogenesis. Therefore, both protection of the diversity of natural grass carp resource and selection of homozygous traits with desired economic genotypes are very important aspects for grass carp breeding.
- grass carp,
- gynogenesis,
- genome,
- microsatellite locus

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References(19)

References

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