A Nucleus-encoded Topological Specificity Factor PpMinE in Physcomitrella patens has Conserved Function Similar to Its Chloroplast-encoded Ancestor

Jiaying Zhu; Weizhong Liu; Weiwei Zhou; Yong Hu; Yikun He

doi:10.1016/S1673-8527(07)60024-1

Volume 34 Issue 3

Mar. 2007

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Article Navigation > Journal of Genetics and Genomics > 2007 > 34(3): 229-238

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A Nucleus-encoded Topological Specificity Factor PpMinE in Physcomitrella patens has Conserved Function Similar to Its Chloroplast-encoded Ancestor

doi: 10.1016/S1673-8527(07)60024-1

a
College of Life Science, Capital Normal University, Beijing 100037, China
b
College of Life Science, Shanxi Normal University, Linfen 041004, China

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Corresponding author: E-mail address: yhe@mail.cnu.edu.cn (Yikun He)
Received Date: 2006-03-02
Accepted Date: 2006-07-28

Available Online: 2007-05-09

Publish Date: 2007-03-20

Abstract

Abstract

A nucleus-encoded MinE gene, designated PpMinE, from Physcomitrella patens was identified using RT-PCR. The presence of both N- and C-terminal extensions in PpMinE protein suggested its cyanobacterial origin. The transient expression of PpMinE using green fluorescent protein fusion in tobacco (Nicotiana tabacum L.) indicated that the PpMinE was a chloroplast-targeted protein. Overexpression of PpMinE in Escherichia coli caused division site misplacement and minicell formation, suggesting evolutionary functional conservation of MinE during plant phylogenesis. According to the phylogenetic tree, PpMinE protein has a close relationship with the highland plants, which suggests that the transfer events of MinE gene from plastid to nucleus might have occurred before the origin of the land plants.
- plastid division,
- PpMinE,
- lateral gene transfer (LGT),
- evolution,
- transient expression

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References(30)

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