Journal of Genetics and Genomics

A zebrafish gene trap line expresses GFP recapturing expression pattern of foxj1b

Tian Tian, Long Zhao, Xinyi Zhao, Min Zhang, Anming Meng

2009, 36(10): 581-589. doi: 10.1016/S1673-8527(08)60150-2

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Abstract:
Foxj1 has been found to play an important role in cilia formation and function in vertebrates. The zebrafish or Xenopus genome expresses two Foxj1 genes, foxj1a/FoxJ1 and foxj1b/FoxJ1.2. In this study, we have generated a zebrafish transgenic line T2BGSZ10 by To l 2 transposon-based gene trapping ap...

Bioinformatic analysis of microRNA biogenesis and function related proteins in eleven animal genomes

Xiuying Liu, GuanZheng Luo, Xiujuan Bai, Xiu-Jie Wang

2009, 36(10): 591-601. doi: 10.1016/S1673-8527(08)60151-4

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Abstract:
MicroRNAs are ∼22 nt long small non-coding RNAs that play important regulatory roles in eukaryotes. The biogenesis and functional processes of microRNAs require the participation of many proteins, of which, the well studied ones are Dicer, Drosha, Argonaute and Exportin 5. To systematically study th...

Evolution of double MutT/Nudix domain-containing proteins: similar domain architectures from independent gene duplication-fusion events

Jun Lin, Yihuai Hu, Bing Tian, Yuejin Hua

2009, 36(10): 603-610. doi: 10.1016/S1673-8527(08)60152-6

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Abstract:
The MutT/Nudix superfamily proteins repair DNA damage and play a role in human health and disease. In this study, we examined two different cases of double MutT/Nudix domain-containing proteins from eukaryotes and prokaryotes. Firstly, these double domain proteins were discovered in Drosophila, but ...

Analysis of expressed receptor-like kinases (RLKs) in soybean

Peng Liu, Wei Wei, Shouqiang Ouyang, Jin-Song Zhang, Shou-Yi Chen, Wan-Ke Zhang

2009, 36(10): 611-619. doi: 10.1016/S1673-8527(08)60153-8

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Abstract:
Receptor-like kinases (RLKs) play crucial roles in cellular signal perception and propagation. To study the evolutionary relationships among RLKs in soybean, a large-scale expressed sequence tags (ESTs) survey for RLKs-related sequences was conducted. By doing BLAST analysis using our database and T...

Distinguishing transgenic from non-transgenic Arabidopsis plants by 1H NMR-based metabolic fingerprinting

Yanfei Ren, Tao Wang, Yufa Peng, Bin Xia, Li-Jia Qu

2009, 36(10): 621-628. doi: 10.1016/S1673-8527(08)60154-X

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Abstract:
We have recently reported the construction of an nuclear magnetic resonance (NMR)-based metabonomics study platform, Automics. To examine the application of Automics in transgenic plants, we performed metabolic fingerprinting analysis, i.e., ¹H NMR spectroscopy and multivariate analysis, on wild-typ...

A comparative proteomics approach to detect unintended effects in transgenic Arabidopsis

Yanfei Ren, Jun Lv, Hua Wang, Linchuan Li, Yufa Peng, Li-Jia Qu

2009, 36(10): 629-639. doi: 10.1016/S1673-8527(08)60155-1

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Abstract:
Recently, as part of biosafety assessments, unintended effects have been given much attention. In this study, we applied a proteomics approach to elucidate the unintended effects of random T-DNA insertion in transgenic plants. Separated proteins extracted from 12 trans-genic Arabidopsis thaliana wit...

Instructions for authors

2009, 36(10): 641-644. doi: 10.1016/S1673-8527(09)60019-9

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Abstract:

2009 Vol. 36, No. 10