Epigenomic features associated with body temperature stabilize tissues during cold exposure in cold-resistant pigs

Yaping Guo; Mingyang Hu; Hao Peng; Yan Zhang; Renzhuo Kuang; Zheyu Han; Daoyuan Wang; Yinlong Liao; Ruixian Ma; Zhixiang Xu; Jiahao Sun; Yu Shen; Changzhi Zhao; Hong Ma; Di Liu; Shuhong Zhao; Yunxia Zhao

doi:10.1016/j.jgg.2024.06.017

Volume 51 Issue 11

Nov. 2024

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Article Navigation > Journal of Genetics and Genomics > 2024 > 51(11): 1252-1264

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Epigenomic features associated with body temperature stabilize tissues during cold exposure in cold-resistant pigs

doi: 10.1016/j.jgg.2024.06.017

a. Key Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction of the Ministry of Education and Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture, Huazhong Agricultural University, Wuhan, Hubei 430070, China;
b. Yazhouwan National Laboratory, Sanya, Hainan 572000, China;
c. Institute of Animal Husbandry, Heilongjiang Academy of Agricultural Sciences, Harbin, Heilongjiang 150086, China;
d. The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, Hubei 430070, China

Funds:

This work was supported by the National Natural Science Foundation of China (32202634), National Natural Science Foundation of China (31961143020), National Natural Science Foundation of China (U20A2052), China Agricultural Research System (CARS-35), and the Heilongjiang Provincial Research Institutes Research Business Fund Project (CZKYF2023-1-B009).

Received Date: 2024-03-27
Accepted Date: 2024-06-27
Rev Recd Date: 2024-06-26

Available Online: 2025-06-06

Publish Date: 2024-07-04

Abstract

Abstract

Cold stress in low-temperature environments can trigger changes in gene expression, but epigenomics regulation of temperature stability in vital tissues, including the fat and diencephalon, is still unclear. Here, we explore the cold-induced changes in epigenomic features in the diencephalon and fat tissues of two cold-resistant Chinese pig breeds, Min and Enshi black (ES) pigs, utilizing H3K27ac CUT&Tag, RNA-seq, and selective signature analysis. Our results show significant alterations in H3K27ac modifications in the diencephalon of Min pigs and the fat of ES pigs after cold exposure. Dramatic changes in H3K27ac modifications in the diencephalon of Min pig are primarily associated with genes involved in energy metabolism and hormone regulation, whereas those in the fat of ES pig are primarily associated with immunity-related genes. Moreover, transcription factors PRDM1 and HSF1, which show evidence of selection, are enriched in genomic regions presenting cold-responsive alterations in H3K27ac modification in the Min pig diencephalon and ES pig fat, respectively. Our results indicate the diversity of epigenomic response mechanisms to cold exposure between Min and ES pigs, providing unique epigenetic resources for studies of low-temperature adaptation in large mammals.
- Cold exposure,
- Diencephalon tissue,
- Fat tissue,
- H3K27ac modification,
- Breed difference,
- Multi-omics

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References(61)

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