Nitrogen inhibition of nitrogenase activity involves the modulation of cytosolic invertase in soybean nodule

Qinzhen Xu; Xin Wang; Nan Wang; Suning Li; Xiaolei Yao; Huaqin Kuang; Zhimin Qiu; Danxia Ke; Wenqiang Yang; Yuefeng Guan

doi:10.1016/j.jgg.2024.06.013

Volume 51 Issue 12

Dec. 2024

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Article Navigation > Journal of Genetics and Genomics > 2024 > 51(12): 1404-1412

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Nitrogen inhibition of nitrogenase activity involves the modulation of cytosolic invertase in soybean nodule

doi: 10.1016/j.jgg.2024.06.013

a. College of Resources and Environment, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China;
b. Photosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China;
c. Guangdong Provincial Key Laboratory of Plant Adaptation and Molecular Design, Innovative Center of Molecular Genetics and Evolution, School of Life Sciences, Guangzhou University, Guangzhou, Guangdong 510006, China;
d. School of Life Sciences, Inner Mongolia University, Hohhot, Inner Mongolia 010000, China;
e. Jiangxi Province Key Laboratory of Oil Crops Genetic Improvement (2024SSY04031), Crop Institute, Jiangxi Academy of Agricultural Sciences, Nanchang, Jiangxi 330200, China;
f. Zhejiang Institute of Subtropical Crops, Zhejiang Academy of Agricultural Sciences, Wenzhou, Zhejiang 325005, China;
g. College of Life Sciences & Institute for Conservation and Utilization of Agro-bioresources in Dabie Mountains, Xinyang Normal University, Xinyang, Henan 464000, China

Funds:

This work was supported by funds from the National Natural Science Foundation of China (32072083) and a Guangzhou Science Grant (2024A03J0010) awarded to Y.G.

Received Date: 2024-03-08
Accepted Date: 2024-06-24
Rev Recd Date: 2024-06-23

Available Online: 2025-06-05

Publish Date: 2024-06-29

Abstract

Abstract

Legume symbiotic nitrogen fixation (SNF) is suppressed by inorganic nitrogen (N) in the soil. High N inhibition of nitrogenase activity is associated with the deprivation of carbon allocation and metabolism in nodules. However, the underlying molecular mechanisms remain unclear. Here, we identify GmCIN1, which encodes a cytosolic invertase, as a gateway for the N-tuning of sucrose utilization in nodules. GmCIN1 is enriched in mature soybean nodules, and its expression is regulated by nitrogen status. The knockout of GmCIN1 using genome editing partially mimics the inhibitory effects of N on nitrogenase activity and sugar content and the impact of high N on nodule transcriptomes. This indicates that GmCIN1 partially mediates the high N inhibition of nodule activity. Moreover, ChIP-qPCR and EMSA reveal that SNAP1/2 transcription factors directly bind to the GmCIN1 promoter. In addition, SNAP1/2 may be involved in the repression of GmCIN1 expression in mature nodules at high N concentrations. Our findings provide insights into the involvement of the transcriptional tuning of carbon (C) metabolism genes by N-signaling modulators in the N-induced inhibition of nitrogenase activity.
- Cytosolic invertase,
- GmCIN1,
- Nitrogen fixation,
- Carbon metabolism,
- Symbiotic nitrogen fixation,
- Legume

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References

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