Dosage effect genes modulate grain development in synthesized <i>Triticum durum</i>-<i>Haynaldia villosa</i> allohexaploid

Zhongyu Yu; Baofeng Cui; Jin Xiao; Wu Jiao; Haiyan Wang; Zongkuan Wang; Li Sun; Qingxin Song; Jingya Yuan; Xiue Wang

doi:10.1016/j.jgg.2024.04.010

Volume 51 Issue 10

Oct. 2024

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Article Navigation > Journal of Genetics and Genomics > 2024 > 51(10): 1089-1100

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Dosage effect genes modulate grain development in synthesized Triticum durum-Haynaldia villosa allohexaploid

doi: 10.1016/j.jgg.2024.04.010

State Key Lab of Crop Genetics & Germplasm Enhancement and Utilization, Cytogenetics Institute, Nanjing Agricultural University/JCIC-MCP/Zhongshan Biological Breeding Laboratory, Nanjing, Jiangsu 210095, China

Funds:

This work was financially supported by the National Key Research and Development Program of China (Grant No. 2022YFF1002900 and 2020YFE0202900), the National Natural Science Foundation of China (32270576), Jiangsu Provincial Key Research and Development Program (BE2022346), Seed Industry Revitalization Project of Jiangsu Province (JBGS[2021]006 and JBGS[2021]013), the Jiangsu Agricultural Technology System (JATS[2023]422), the Joint Research of Wheat Variety Improvement of Anhui, and Zhongshan Biological Breeding Laboratory (ZSBBL) (ZSBBL-KY2023-02-2).

Received Date: 2023-09-02
Accepted Date: 2024-04-15
Rev Recd Date: 2024-04-15

Available Online: 2025-06-06

Publish Date: 2024-04-25

Abstract

Abstract

Polyploidization in plants often leads to increased cell size and grain size, which may be affected by the increased genome dosage and transcription abundance. The synthesized Triticum durum (AABB)-Haynaldia villosa (VV) amphiploid (AABBVV) has significantly increased grain size, especially grain length, than the tetraploid and diploid parents. To investigate how polyploidization affects grain development at the transcriptional level, we perform transcriptome analysis using the immature seeds of T. durum, H. villosa, and the amphiploid. The dosage effect genes are contributed more by differentially expressed genes from genome V of H. villosa. The dosage effect genes overrepresent grain development-related genes. Interestingly, the vernalization gene TaVRN1 is among the positive dosage effect genes in the T. durum-H. villosa and T. turgidum-Ae. tauschii amphiploids. The expression levels of TaVRN1 homologs are positively correlated with the grain size and weight. The TaVRN1-B1 or TaVRN1-D1 mutation shows delayed florescence, decreased cell size, grain size, and grain yield. These data indicate that dosage effect genes could be one of the important explanations for increased grain size by regulating grain development. The identification and functional validation of dosage effect genes may facilitate the finding of valuable genes for improving wheat yield.
- Allopolyploid,
- Polyploidization,
- Dosage effect,
- Wheat,
- Grain size,
- TaVRN1

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References(72)

References

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