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Volume 51 Issue 7
Jul.  2024
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Article Navigation >  Journal of Genetics and Genomics  > 2024  >  51(7): 749-761

Root-derived long-distance signals trigger ABA synthesis and enhance drought resistance in Arabidopsis

doi: 10.1016/j.jgg.2024.03.007
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    a. Division of Life Sciences and Medicine, Division of Molecular & Cell Biophysics, Hefei National Science Center for Interdisciplinary Sciences at the Microscale, University of Science and Technology of China, The Innovation Academy of Seed Design, Chinese Academy of Sciences, Hefei, Anhui 230027, China;

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    b. College of Life Sciences, Anhui Agricultural University, Hefei, Anhui 230036, China;

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    c. College of Agronomy, South China Agricultural University, Guangzhou, Guangdong 510642, China;

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    d. School of Biology and Basic Medical Sciences, Soochow University, Suzhou, Jiangsu 215123, China

Funds:

This research was supported by grants from the National Natural Science Foundation of China (31900230 to P.X.Z.) and the China Postdoctoral Science Foundation (2020T130634 and 2019M652200 to P.X.Z.).

  • Received Date: 2024-03-21
  • Accepted Date: 2024-03-25
  • Rev Recd Date: 2024-03-24
    • Available Online: 2025-06-06
  • Publish Date: 2024-03-28
    Abstract
  • Vascular plants have evolved intricate long-distance signaling mechanisms to cope with environmental stress, with reactive oxygen species (ROS) emerging as pivotal systemic signals in plant stress responses. However, the exact role of ROS as root-to-shoot signals in the drought response has not been determined. In this study, we reveal that compared with wild-type plants, ferric reductase defective 3 (frd3) mutants exhibit enhanced drought resistance concomitant with elevated NINE-CIS-EPOXYCAROTENOID DIOXYGENASE 3 (NCED3) transcript levels and abscisic acid (ABA) contents in leaves as well as increased hydrogen peroxide (H2O2) levels in roots and leaves. Grafting experiments distinctly illustrate that drought resistance can be conferred by the frd3 rootstock regardless of the scion genotype, indicating that long-distance signals originating from frd3 roots promote an increase in ABA levels in leaves. Intriguingly, the drought resistance conferred by the frd3 mutant rootstock is weakened by the CAT2-overexpressing scion, suggesting that H2O2 may be involved in long-distance signaling. Moreover, the results of comparative transcriptome and proteome analyses support the drought resistance phenotype of the frd3 mutant. Taken together, our findings substantiate the notion that frd3 root-derived long-distance signals trigger ABA synthesis in leaves and enhance drought resistance, providing new evidence for root-to-shoot long-distance signaling in the drought response of plants.
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