The CCT transcriptional activator Ghd2 constantly delays the heading date by upregulating <i>CO3</i> in rice

Xiaowei Fan; Pengfei Wang; Feixiang Qi; Yong Hu; Shuangle Li; Jia Zhang; Liwen Liang; Zhanyi Zhang; Juhong Liu; Lizhong Xiong; Yongzhong Xing

doi:10.1016/j.jgg.2023.03.002

Volume 50 Issue 10

Oct. 2023

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The CCT transcriptional activator Ghd2 constantly delays the heading date by upregulating CO3 in rice

doi: 10.1016/j.jgg.2023.03.002

a. National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, Hubei 430070, China;
b. Hubei Hongshan Laboratory, Wuhan, Hubei 430070, China

Funds:

This study was supported by the Natural Science Foundation of China (U20A2031) and the Earmarked Fund for China Agricultural Research System (CARS-01).

Received Date: 2023-02-27
Accepted Date: 2023-03-03
Rev Recd Date: 2023-03-03
Publish Date: 2023-03-10

Abstract

Abstract

CONSTANS, CO-like, and TOC1 (CCT) family genes play important roles in regulating heading date, which exerts a large impact on the regional and seasonal adaptation of rice. Previous studies have shown that Grain number, plant height, and heading date2 (Ghd2) exhibits a negative response to drought stress by directly upregulating Rubisco activase and exerting a negative effect on heading date. However, the target gene of Ghd2 regulating heading date is still unknown. In this study, CO3 is identified by analyzing Ghd2 ChIP-seq data. Ghd2 activates CO3 expression by binding to the CO3 promoter through its CCT domain. EMSA experiments show that the motif CCACTA in the CO3 promoter was recognized by Ghd2. A comparison of the heading dates among plants with CO3 knocked out or overexpressed and double-mutants with Ghd2 overexpressed and CO3 knocked out shows that CO3 negatively and constantly regulates flowering by repressing the transcription of Ehd1, Hd3a, and RFT1. In addition, the target genes of CO3 are explored via a comprehensive analysis of DAP-seq and RNA-seq data. Taken together, these results suggest that Ghd2 directly binds to the downstream gene CO3, and the Ghd2-CO3 module constantly delays heading date via the Ehd1-mediated pathway.
- Heading date,
- CCT family gene,
- ChIP-seq,
- Constant repressor,
- Rice (Oryza sativa L.)

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