Generation of <i>Plvap-CreER</i> and <i>Car4-CreER</i> for genetic targeting of distinct lung capillary populations

Zhenqian Zhang; Bin Zhou

doi:10.1016/j.jgg.2022.08.001

Volume 49 Issue 12

Dec. 2022

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Article Navigation > Journal of Genetics and Genomics > 2022 > 49(12): 1093-1100

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Generation of Plvap-CreER and Car4-CreER for genetic targeting of distinct lung capillary populations

doi: 10.1016/j.jgg.2022.08.001

Zhenqian Zhang^a,
Bin Zhou^a,b,c

a. State Key Laboratory of Cell Biology, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences; University of Chinese Academy of Sciences, Shanghai 200031, China;
b. Key Laboratory of Systems Health Science of Zhejiang Province, School of Life Science, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou, Zhejiang 310024, China;
c. School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China

Funds:

This study was supported by the National Key Research & Development Program of China (2019YFA0110403, 2019YFA080200), the National Science Foundation of China (82088101, 32050087, 91849202, 31730112).

Received Date: 2022-01-26
Accepted Date: 2022-08-01
Rev Recd Date: 2022-08-01
Publish Date: 2022-08-23

Abstract

Abstract

It has been reported recently that there are two distinct subpopulations of capillary endothelial cells in the mammalian lungs: gCap (general capillary) and aCap (aerocyte). They are identified by two unique markers, respectively: plasmalemmal vesicle-associated protein (PLVAP) and carbonic anhydrase IV (CAR4). Here, we report two novel knock-in mouse lines Plvap-CreER and Car4-CreER, which genetically target gCap and aCap, respectively. Induced by tamoxifen, the Plvap-CreER and Car4-CreER alleles mediate specific and efficient Cre-loxP recombinations in PLVAP⁺ gCap and CAR4⁺ aCap, respectively, in the lungs. These two mouse lines are useful genetic tools to investigate cell fates and functions of PLVAP⁺ and CAR4⁺ cells in lung homeostasis, injury and repair.

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References(23)

References

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