Two plant NLR proteins confer strain-specific resistance conditioned by an effector from <i>Pseudomonas syringae</i> pv. <i>actinidiae</i>

Xiaojuan Zheng; Zhaoyang Zhou; Zhen Gong; Meijuan Hu; Ye Jin Ahn; Xiaojuan Zhang; Yan Zhao; Guoshu Gong; Jian Zhang; Jianru Zuo; Guan-Zhu Han; Sohn Kee Hoon; Jian-Min Zhou

doi:10.1016/j.jgg.2022.06.006

Volume 49 Issue 8

Aug. 2022

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Article Navigation > Journal of Genetics and Genomics > 2022 > 49(8): 823-832

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Two plant NLR proteins confer strain-specific resistance conditioned by an effector from Pseudomonas syringae pv. actinidiae

doi: 10.1016/j.jgg.2022.06.006

a. State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing 100101, China;
b. CAS Center for Excellence in Biotic Interactions, University of Chinese Academy of Sciences, Beijing 100049, China;
c. College of Horticulture, China Agricultural University, Beijing 100193, China;
d. College of Life Sciences, Jiangsu Key Laboratory for Microbes and Functional Genomics, Nanjing Normal University, Nanjing, Jiangsu 210023, China;
e. Department of Life Sciences, Pohang University of Science and Technology, Pohang 37673, Republic of Korea;
f. School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology, Pohang 37673, Republic of Korea;
g. Plant Protection Department and Major Crop Disease Laboratory, College of Agronomy, Sichuan Agricultural University, Chengdu, Sichuan 611130, China;
h. Hainan Yazhou Bay Seed Laboratory, Sanya, Hainan 572025, China

Funds:

D Program of China (2021YFA1300701) to J.M.Z., the National Natural Science Foundation of China (31872654) to Z.Y.Z., and the Hainan Excellent Talent Team, and the State Key Laboratory of Plant Genomics (SKLPG2016B-2) to J.M.Z.

We thank Yuxin Hu for the sze1 sze2 mutant and Brian Staskawicz for the Nbzar1 mutant. We thank Qi-Jun Chen for providing the CRISPR/Cas9 vectors. The work was supported by grants from the National Key R&

Received Date: 2022-05-13
Accepted Date: 2022-06-15
Rev Recd Date: 2022-06-06
Publish Date: 2022-06-24

Abstract

Abstract

Pseudomonas syringae pv. actinidiae (Psa) causes bacterial canker, a devastating disease threatening the Actinidia fruit industry. In a search for non-host resistance genes against Psa, we find that the nucleotide-binding leucine-rich repeat receptor (NLR) protein ZAR1 from both Arabidopsis and Nicotiana benthamiana (Nb) recognizes HopZ5 and triggers cell death. The recognition requires ZED1 in Arabidopsis and JIM2 in Nb plants, which are members of the ZRK pseudokinases and known components of the ZAR1 resistosome. Surprisingly, Arabidopsis ZAR1 and RPM1, another NLR known to recognize HopZ5, confer disease resistance to HopZ5 in a strain-specific manner. Thus, ZAR1, but not RPM1, is solely required for resistance to P.s. maculicola ES4326 (Psm) carrying hopZ5, whereas RPM1 is primarily required for resistance to P.s. tomato DC3000 (Pst) carrying hopZ5. Furthermore, the ZAR1-mediated resistance to Psm hopZ5 in Arabidopsis is insensitive to SOBER1, which encodes a deacetylase known to suppress the RPM1-mediated resistance to Pst hopZ5. In addition, hopZ5 enhances P.syringae virulence in the absence of ZAR1 or RPM1 and that SOBER1 abolishes such virulence function. Together the study suggests that ZAR1 may be used for improving Psa resistance in Actinidia and uncovers previously unknown complexity of effector-triggered immunity and effector-triggered virulence.
- NLR,
- ZAR1,
- Pseudomonas syringae pv. actinidiae,
- Immunity,
- Disease resistance

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References(53)

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