Cryo-EM structure of R-loop monoclonal antibody S9.6 in recognizing RNA: DNA hybrids

Qin Li; Chao Lin; Zhipu Luo; Haitao Li; Xueming Li; Qianwen Sun

doi:10.1016/j.jgg.2022.04.011

Volume 49 Issue 7

Jul. 2022

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Article Navigation > Journal of Genetics and Genomics > 2022 > 49(7): 677-680

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Cryo-EM structure of R-loop monoclonal antibody S9.6 in recognizing RNA: DNA hybrids

doi: 10.1016/j.jgg.2022.04.011

1. Center for Plant Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China;
2. Beijing Advanced Innovation Center for Structural Biology, Tsinghua University, Beijing 100084, China;
3. Institute of Molecular Enzymology, School of Biology and Basic Medical Sciences, Soochow University, Suzhou, Jiangsu 215123, China;
4. Beijing Advanced Innovation Center for Structural Biology, Tsinghua University, Beijing 100084, China;
5. Tsinghua-Peking Center for Life Sciences, Beijing 100084, China;
6. Center for Plant Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China;
7. Tsinghua-Peking Center for Life Sciences, Beijing 100084, China

Funds:

We thank Dr. Jun-Jie G. Liu, S. Fan and L. Zhang for useful discussions and suggestions

the Beijing Advanced Innovation Center for Structural Biology for facility and financial support. This work was supported by grants from Beijing Advanced Innovation Center for Structural Biology (Number 041301470). The Q. Sun Lab, X. Li Lab and H. Li Lab are supported by Tsinghua-Peking Center for Life Sciences, China.

the Center of Biomedical Analysis in Tsinghua University, China for affinity detection of S9.6

J. Lei and X. Li for technical help. We thank the Protein Preparation and Identification Facility at Technology Center for Protein Science in Tsinghua University, China for purification of S9.6

Received Date: 2022-04-11
Accepted Date: 2022-04-24
Rev Recd Date: 2022-04-21
Publish Date: 2022-05-10

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References(17)

References

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