The SNAPc complex mediates starvation-induced trans-splicing in <i>Caenorhabditis elegans</i>

Xinhao Hou; Chengming Zhu; Mingjing Xu; Xiangyang Chen; Cheng Sun; Bj&#246;rn Nashan; Shouhong Guang; Xuezhu Feng

doi:10.1016/j.jgg.2022.02.024

Volume 49 Issue 10

Oct. 2022

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Article Navigation > Journal of Genetics and Genomics > 2022 > 49(10): 952-964

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The SNAPc complex mediates starvation-induced trans-splicing in Caenorhabditis elegans

doi: 10.1016/j.jgg.2022.02.024

a. The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, School of Life Sciences, Ministry of Education Key Laboratory for Membraneless Organelles & Cellular Dynamics, The USTC RNA Institute, Department of Obstetrics and Gynecology, Biomedical Sciences and Health Laboratory of Anhui Province, University of Science and Technology of China, Hefei, Anhui 230001, China;
b. CAS Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, Hefei, Anhui 230027, China

Funds:

D Program of China (2019YFA0802600), the Strategic Priority Research Program of the Chinese Academy of Sciences (XDB39010600), the National Natural Science Foundation of China (91940303, 31870812, 32070619, 31871300 and 31900434), the China Postdoctoral Science Foundation (2018M632542), and the Anhui Natural Science Foundation (1808085QC82 and 1908085QC96). This study was supported in part by the Fundamental Research Funds for the Central Universities.

We are grateful to the members of the Guang lab for their comments. We are also grateful to the International C. elegans Gene Knockout Consortium and the National Bioresource Project for providing the strains. Some strains were provided by the CGC, which is funded by the NIH Office of Research Infrastructure Programs (P40 OD010440). This work was supported by grants from the National Key R&

Received Date: 2021-11-14
Accepted Date: 2022-02-22
Rev Recd Date: 2022-02-20
Publish Date: 2022-03-10

Abstract

Abstract

Dietary restriction usually suppresses biosynthesis but activates catabolic pathways in animals. However, the short-term starvation enhances biosynthetic activities and promotes ribosomal biogenesis in adult Caenorhabditis elegans. The mechanism underlying the processes remains largely unknown. Here, we find that the short-term starvation enhances the SL1 trans-splicing of translation-related genes in adult C. elegans by transcriptome analysis. The small nuclear RNA-activating protein complex (SNAPc) promotes SL RNA production and mediates starvation-induced trans-splicing. TOFU-5, a core factor in the upstream sequence transcription complex (USTC) essential for piRNA production, is also involved in the starvation-induced trans-splicing processes. Knocking down components of the SNAPc complex and tofu-5 extends worm survival under starvation conditions. Taken together, our study highlights the importance of SL trans-splicing in the nutrition response and reveals a mechanism of the survival regulation by food deprivation via SNAPc and TOFU-5.
- Trans-splicing,
- SNAPc,
- tofu-5,
- Starvation,
- C. elegans

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References(55)

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