Function of hydroxycinnamoyl transferases for the biosynthesis of phenolamides in rice resistance to <i>Magnaporthe oryzae</i>

Hong Fang; Fan Zhang; Chongyang Zhang; Dan Wang; Shuangqian Shen; Feng He; Hui Tao; Ruyi Wang; Min Wang; Debao Wang; Xionglun Liu; Jie Luo; Guo-Liang Wang; Yuese Ning

doi:10.1016/j.jgg.2022.02.008

Volume 49 Issue 8

Aug. 2022

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Article Navigation > Journal of Genetics and Genomics > 2022 > 49(8): 776-786

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Function of hydroxycinnamoyl transferases for the biosynthesis of phenolamides in rice resistance to Magnaporthe oryzae

doi: 10.1016/j.jgg.2022.02.008

a. College of Agronomy, Hunan Agricultural University, Changsha, Hunan 410128, China;
b. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
c. National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan, Hubei 430070, China;
d. College of Tropical Crops, Hainan University, Haikou, Hainan 572208, China;
e. Department of Plant Pathology, The Ohio State University, Columbus, OH 43210, USA

Funds:

This work was supported by grants from the National Natural Science Foundation of China (32161143009, 31822041 and 31972225) to Y.N., the National Natural Science Foundation of China (U20A2021) to R.W. and the National Natural Science Foundation of China (31801692) to F.Z.

Received Date: 2021-11-13
Accepted Date: 2022-02-06
Rev Recd Date: 2022-02-04
Publish Date: 2022-02-26

Abstract

Abstract

Phenolamide (PA) metabolites play important roles in the interaction between plants and pathogens. The putrescine hydroxycinnamoyl transferase genes OsPHT3 and OsPHT4 positively regulate rice cell death and resistance to Magnaporthe oryzae. The bZIP transcription factor APIP5, a negative regulator of cell death and rice immunity, directly binds to the OsPHT4 promoter to regulate putrescine-derived PAs. Whether other hydroxycinnamoyl transferase (HT) genes also participate in APIP5-mediated immunity remains unclear. Surprisingly, we find that genes encoding agmatine hydroxycinnamoyl transferases OsAHT1 and OsAHT2, tryptamine hydroxycinnamoyl transferases OsTBT1 and OsTBT2, and tyramine hydroxycinnamoyl transferases OsTHT1 and OsTHT2, responsible for the biosynthesis of polyamine-derived PAs are all up-regulated in APIP5-RNAi transgenic plants compared with segregated wild-type rice. Furthermore, both OsAHT1/2 and OsTBT1/2 are induced during M.oryzae infection, showing expression patterns similar to those previously reported for OsTHT1/2 and OsPHT3/4. Transgenic plants overexpressing either OsAHT2-GFP or OsTBT1-GFP show enhanced resistance against M.oryzae and accumulated more PA metabolites and lignin compared with wild-type plants. Interestingly, as demonstrated for OsPHT4, APIP5 directly binds to the promoters of OsAHT1/2, OsTBT1/2, and OsTHT1/2, repressing their transcription. Together, these results indicate that the HT genes are common targets of APIP5 and that PAs play critical roles in rice immunity.
- Hydroxycinnamoyl transferases,
- Phenolamides,
- Magnaporthe oryzae,
- bZIP transcription factor,
- Lignin

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References

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