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Volume 49 Issue 5
May  2022
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Article Navigation >  Journal of Genetics and Genomics  > 2022  >  49(5): 414-426

OsbZIP60-mediated unfolded protein response regulates grain chalkiness in rice

doi: 10.1016/j.jgg.2022.02.002
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    a National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan, Hubei 430070, China;

  •  

    b Hubei Hongshan Laboratory, Wuhan, Hubei 430070, China

Funds:

This study is supported by grants from the National Key Research and Development Program of China (2021YFF1000202), the National Natural Science Foundation of China (31771368, 31821005, 31701397), Ten-thousand Talents Programs of China, Fundamental Research Funds for the Central Universities (2662020SKPY006), Wuhan Applied Foundational Frontier Project (2020020601012257) and Hubei Hongshan Laboratory (2021hszd005), China.

  • Received Date: 2021-10-30
  • Accepted Date: 2022-02-07
  • Rev Recd Date: 2022-02-01
  • Publish Date: 2022-02-19
    Abstract
  • Grain chalkiness, an undesirable trait caused by complex factors, has great negative impacts on the quality and economic value of rice. However, little is known about the regulatory mechanism of grain chalkiness, particularly the effect of endoplasmic reticulum (ER) stress. Here, a genome-wide association study (GWAS) reveals that the transcription factor OsbZIP60 is a vital regulator of rice grain chalkiness. Genetic analysis demonstrates that knockout of OsbZIP60 results in extremely high grain chalkiness and aberrant structure of storage substances. Notably, the expression of unfolded protein response (UPR) genes, such as OsbZIP50, OsBiP1, OsBiP2 and OsBiP3, is up-regulated in the endosperm cells of osbzip60, and overexpression of all these UPR genes causes various degrees of chalkiness. Furthermore, OsbZIP60 is found to activate the expression of key genes related to grain chalkiness, such as GPA3, FSE1, FLO7, Chalk5, OsNF-YB1, and OsPK2, whose expression is significantly suppressed in osbzip60 and overexpression lines of OsbZIP50, OsBiP1, OsBiP2, and OsBiP3. Our study provides novel insights into the function of OsbZIP60 and the role of the UPR pathway in the formation of grain chalkiness in rice.
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