The shikimate pathway regulates programmed cell death

Xuerui Lu; Shixi Shi; Chong Wu; Xueao Zheng; Chenkun Yang; Jie Luo; Shunping Yan

doi:10.1016/j.jgg.2022.02.001

Volume 49 Issue 10

Oct. 2022

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The shikimate pathway regulates programmed cell death

doi: 10.1016/j.jgg.2022.02.001

Xuerui Lu^a,b,
Shixi Shi^a,b,
Chong Wu^a,b,
Xueao Zheng^a,b,
Chenkun Yang^b,c,
Jie Luo^b,c,
Shunping Yan^a,b

a. Hubei Hongshan Laboratory, Wuhan, Hubei 430070, China;
b. College of Life Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China;
c. National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, Hubei 430070, China

Funds:

Technological Self-innovation Foundation (2014RC004).

We thank Dr. Ting Pan for the helpful discussion and Dr. Lianghuan Qu for the help in metabolic analysis. This work was supported by grants from the National Natural Science Foundation of China (31771355 and 31970311), Thousand Talents Plan of China-Young Professionals, and Huazhong Agricultural University Scientific &

Received Date: 2022-01-24
Accepted Date: 2022-02-02
Rev Recd Date: 2022-01-28
Publish Date: 2022-02-12

Abstract

Abstract

Programmed cell death (PCD) is essential for both plant development and stress responses including immunity. However, how plants control PCD is not well-understood. The shikimate pathway is one of the most important metabolic pathways in plants, but its relationship to PCD is unknown. Here, we show that the shikimate pathway promotes PCD in Arabidopsis. We identify a photoperiod-dependent lesion-mimic mutant named Lesion in short-day (lis), which forms spontaneous lesions in short-day conditions. Map-based cloning and whole-genome resequencing reveal that LIS encodes MEE32, a bifunctional enzyme in the shikimate pathway. Metabolic analysis shows that the level of shikimate is dramatically increased in lis. Through genetic screenings, three suppressors of lis (slis) are identified and the causal genes are cloned. SLISes encode proteins upstream of MEE32 in the shikimate pathway. Furthermore, exogenous shikimate treatment causes PCD. Our study uncovers a link between the shikimate pathway and PCD, and suggests that the accumulation of shikimate is an alternative explanation for the action of glyphosate, the most successful herbicide.
- Programmed cell death,
- Shikimate pathway,
- Lesion-mimic mutant,
- Immunity,
- Photoperiod,
- Arabidopsis

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References(48)

References

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