A simple and precise method (Y2H-in-frame-seq) improves yeast two-hybrid screening with cDNA libraries

Yinghui Gu; Guannan Li; Ping Wang; Yan Guo; Jingrui Li

doi:10.1016/j.jgg.2021.11.008

Volume 49 Issue 6

Jun. 2022

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Article Navigation > Journal of Genetics and Genomics > 2022 > 49(6): 595-598

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A simple and precise method (Y2H-in-frame-seq) improves yeast two-hybrid screening with cDNA libraries

doi: 10.1016/j.jgg.2021.11.008

State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100193, China

Funds:

We thank Qi Xie for providing the cDNA library. This work was supported by a grant from the National Natural Science Foundation of China (32170283).

Received Date: 2021-08-05
Revised Date: 2021-11-16
Accepted Date: 2021-11-22
Publish Date: 2022-06-30

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References

Banerjee, S., Velasquez-Zapata, V., Fuerst, G., Elmore, J.M.,Wise, R.P., 2020. NGPINT:a next-generation protein-protein interaction software. Brief Bioinform

Erffelinck, M.L., Ribeiro, B., Perassolo, M., Pauwels, L., Pollier, J., Storme, V.,Goossens, A., 2018. A user-friendly platform for yeast two-hybrid library screening using next generation sequencing. PLoS One 13, e0201270

Fields, S.,Song, O.K., 1989. A Novel Genetic System to Detect Protein Protein Interactions. Nature 340, 245-246

Gong, W., Shen, Y.P., Ma, L.G., Pan, Y., Du, Y.L., Wang, D.H., Yang, J.Y., Hu, L.D., Liu, X.F., Dong, C.X., et al., 2004. Genome-wide ORFeome cloning and analysis of Arabidopsis transcription factor genes. Plant Physiol 135, 773-782

Lewis, J.D., Wan, J., Ford, R., Gong, Y., Fung, P., Nahal, H., Wang, P.W., Desveaux, D.,Guttman, D.S., 2012. Quantitative Interactor Screening with next-generation Sequencing (QIS-Seq) identifies Arabidopsis thaliana MLO2 as a target of the Pseudomonas syringae type III effector HopZ2. BMC Genomics 13, 8

Maier, R.H., Maier, C.J.,Onder, K., 2011. Construction of improved Yeast Two-Hybrid libraries. Methods Mol Biol 729, 71-84

Matsuyama, A., Arai, R., Yashiroda, Y., Shirai, A., Kamata, A., Sekido, S., Kobayashi, Y., Hashimoto, A., Hamamoto, M., Hiraoka, Y., et al., 2006. ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe. Nat Biotechnol 24, 841-847

Reboul, J., Vaglio, P., Rual, J.F., Lamesch, P., Martinez, M., Armstrong, C.M., Li, S., Jacotot, L., Bertin, N., Janky, R., et al., 2003. C. elegans ORFeome version 1.1:experimental verification of the genome annotation and resource for proteome-scale protein expression. Nat Genet 34, 35-41

Rual, J.F., Hirozane-Kishikawa, T., Hao, T., Bertin, N., Li, S., Dricot, A., Li, N., Rosenberg, J., Lamesch, P., Vidalain, P.O., et al., 2004. Human ORFeome version 1.1:a platform for reverse proteomics. Genome Res 14, 2128-2135

Trigg, S.A., Garza, R.M., MacWilliams, A., Nery, J.R., Bartlett, A., Castanon, R., Goubil, A., Feeney, J., O'Malley, R., Huang, S.C., et al., 2017. CrY2H-seq:a massively multiplexed assay for deep-coverage interactome mapping. Nat Methods 14, 819-825

Velasquez-Zapata, V., Elmore, J.M., Banerjee, S., Dorman, K.S.,Wise, R.P., 2021. Next-generation yeast-two-hybrid analysis with Y2H-SCORES identifies novel interactors of the MLA immune receptor. PLoS Comput Biol 17, e1008890

Venkatesan, K., Rual, J.F., Vazquez, A., Stelzl, U., Lemmens, I., Hirozane-Kishikawa, T., Hao, T., Zenkner, M., Xin, X., Goh, K.I., et al., 2009. An empirical framework for binary interactome mapping. Nat Methods 6, 83-90

Vidal, M.,Fields, S., 2014. The yeast two-hybrid assay:still finding connections after 25 years. Nat Methods 11, 1203-1206

Weimann, M., Grossmann, A., Woodsmith, J., Ozkan, Z., Birth, P., Meierhofer, D., Benlasfer, N., Valovka, T., Timmermann, B., Wanker, E.E., et al., 2013. A Y2H-seq approach defines the human protein methyltransferase interactome. Nat Methods 10, 339-342

Wierbowski, S.D., Vo, T.V., Falter-Braun, P., Jobe, T.O., Kruse, L.H., Wei, X., Liang, J., Meyer, M.J., Akturk, N., Rivera-Erick, C.A., et al., 2020. A massively parallel barcoded sequencing pipeline enables generation of the first ORFeome and interactome map for rice. Proc Natl Acad Sci U S A 117, 11836-11842

Xie, C., Zhou, X., Deng, X.,Guo, Y., 2010. PKS5, a SNF1-related kinase, interacts with and phosphorylates NPR1, and modulates expression of WRKY38 and WRKY62. J Genet Genomics 37, 359-369

Yang, F., Lei, Y., Zhou, M., Yao, Q., Han, Y., Wu, X., Zhong, W., Zhu, C., Xu, W., Tao, R., et al., 2018. Development and application of a recombination-based library versus library high-throughput yeast two-hybrid (RLL-Y2H) screening system. Nucleic Acids Res 46, e17

Yang, X., Boehm, J.S., Yang, X., Salehi-Ashtiani, K., Hao, T., Shen, Y., Lubonja, R., Thomas, S.R., Alkan, O., Bhimdi, T., et al., 2011. A public genome-scale lentiviral expression library of human ORFs. Nat Methods 8, 659-661

Yu, H., Tardivo, L., Tam, S., Weiner, E., Gebreab, F., Fan, C., Svrzikapa, N., Hirozane-Kishikawa, T., Rietman, E., Yang, X., et al., 2011. Next-generation sequencing to generate interactome datasets. Nat Methods 8, 478-480

Yu, Q., Hu, Y., Su, J., Li, P., Zhang, L., Fu, X., Chen, F.,Song, A., 2020. Evaluation of a Yeast Two-Hybrid Library by High-Throughput Sequencing. J Proteome Res 19, 3567-3572

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