The role of genotype and diet in shaping gut microbiome in a genetic vitamin A deficient mouse model

Jun Xu; Jie-Ni Zhang; Bo-Hui Sun; Qing Liu; Juan Ma; Qian Zhang; Yong-Xin Liu; Ning Chen; Feng Chen

doi:10.1016/j.jgg.2021.08.015

Volume 49 Issue 2

Mar. 2022

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The role of genotype and diet in shaping gut microbiome in a genetic vitamin A deficient mouse model

doi: 10.1016/j.jgg.2021.08.015

a Department of Gastroenterology, Peking University People's Hospital, Beijing 100044, China;
b Clinical Center of Immune-Mediated Digestive Diseases, Peking University People's Hospital, Beijing 100044, China;
c Institute of Clinical Molecular Biology & Central Laboratory, Peking University People's Hospital, Beijing 100044, China;
d Department of Orthodontics, Peking University School of Stomatology, Beijing 100081, China;
e Central Laboratory, Peking University School of Stomatology, Beijing 100081, China;
f CAS Center for Excellence in Biotic Interactions, Chinese Academy of Sciences, Beijing 100049, China

Funds:

This study was funded by the KCL and PKUHSC Joint Institute for Medical Research Fund (BMU2020KCL003), the National Natural Science Foundation of China (No. 81900984, 81991501, 81870747, 82000496, 82070566, and 81870387), the Beijing Municipal Natural Science Foundation (No. 7182184 and 7214267), Capital's Funds for Health Improvement and Research (2020-2Z-40813), and Peking University Clinical Medicine Plus X-Young Scholars Project (PKU2021LCXQ003).

Received Date: 2021-05-05
Accepted Date: 2021-08-26
Rev Recd Date: 2021-08-25
Publish Date: 2021-09-16

Abstract

Abstract

Multifactors have been reported to affect the gut microbiome, including genotype, age, diet, and nutrition. However, few reports have investigated the relative capacity of different factors to shape the gut microbiome in a single study. Our design used a genetic vitamin A-deficient mouse model, the Rbp4 mouse, feeding with the low vitamin A diets at different ages of initiation (4 or 7 weeks) for 28 days. Fecal samples were collected for bacterial profiling at seven time points after diet controlling. With Rbp4 depletion, Akkermansia decreased and Bacteroides increased, whereas Desulfovibrio, Barnesiella, Clostridium_XlVa, and Lactobacillus fluctuated. The bacterial community swiftly adjusted with the vitamin A-deficient diet administration and gradually changed (e.g., decrease of Barnesiella and increase of Desulfovibrio). Age exerted a relatively weaker but long-last influence. At an earlier age to feed a vitamin A-deficient diet, a higher microbial dysbiosis index will be valued. Of note, the shaping effects of diet and age on the bacterial community varied with the difference of genotype, which might indicate a greater role of genotype than diet and age in shaping the gut microbiome.
- Gut microbiome,
- Host genotype,
- Diet,
- Vitamin A deficient,
- Shaping role

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References(49)

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