5.9
CiteScore
5.9
Impact Factor
Volume 48 Issue 11
Nov.  2021
Turn off MathJax
Article Contents

Establishment of transposase-assisted low-input m6A sequencing technique

doi: 10.1016/j.jgg.2021.08.007
Funds:

This study was supported by the Ministry of Science and Technology of China (National Science and Technology Major Project, 2019YFA0802203, 2018YFA0109100), National Natural Science Foundation of China (31922015, 31870808, 91753129), Natural Science Foundation of Guangdong Province (2018B030306044, 2019A1515110099), and China Postdoctoral Science Foundation (2020M672949 and 2019M653164).

  • Received Date: 2021-04-29
  • Accepted Date: 2021-08-10
  • Rev Recd Date: 2021-07-21
  • Publish Date: 2021-11-20
  • loading
  • Abakir, A., Giles, T.C., Cristini, A., Foster, J.M., Dai, N., Starczak, M., RubioRoldan, A., Li, M., Eleftheriou, M., Crutchley, J., et al., 2020. N6-methyladenosine regulates the stability of RNA:DNA hybrids in human cells. Nat. Genet. 52, 48-55.
    Barbieri, I., Tzelepis, K., Pandolfini, L., Shi, J., Millan-Zambrano, G., Robson, S.C., Aspris, D., Migliori, V., Bannister, A.J., Han, N., et al., 2017. Promoter-bound METTL3 maintains myeloid leukaemia by m6A-dependent translation control. Nature 552, 126-131.
    Berg, D.E., Schmandt, M.A., Lowe, J.B., 1983. Specificity of transposon Tn5 insertion. Genetics 105, 813-828.
    Boccaletto, P., Machnicka, M.A., Purta, E., Piatkowski, P., Baginski, B., Wirecki, T.K., de Crecy-Lagard, V., Ross, R., Limbach, P.A., Kotter, A., et al., 2018. MODOMICS:a database of RNA modification pathways. 2017 update. Nucleic Acids Res. 46, D303-D307.
    Di, L., Fu, Y., Sun, Y., Li, J., Liu, L., Yao, J., Wang, G., Wu, Y., Lao, K., Lee, R.W., et al., 2020. RNA sequencing by direct tagmentation of RNA/DNA hybrids. Proc. Natl. Acad. Sci. U. S. A. 117, 2886-2893.
    Dominissini, D., Moshitch-Moshkovitz, S., Schwartz, S., Salmon-Divon, M., Ungar, L., Osenberg, S., Cesarkas, K., Jacob-Hirsch, J., Amariglio, N., Kupiec, M., et al., 2012. Topology of the human and mouse m6A RNA methylomes revealed by m6A-seq. Nature 485, 201-206.
    Goodwin, G.H., Sanders, C., Johns, E.W., 1973. A new group of chromatinassociated proteins with a high content of acidic and basic amino acids. Eur. J. Biochem. 38, 14-19.
    Liu, N., Wu, Y., Wen, X., Li, P., Lu, F., Shang, H., 2021. Chronic stress promotes acute myeloid leukemia progression through HMGB1/NLRP3/IL-1β signaling pathway. J. Mol. Med. (Berl.) 99, 403-414.
    Liu, T., Wei, Q., Jin, J., Luo, Q., Liu, Y., Yang, Y., Cheng, C., Li, L., Pi, J., Si, Y., et al., 2020. The m6A reader YTHDF1 promotes ovarian cancer progression via augmenting EIF3C translation. Nucleic Acids Res. 48, 3816-3831.
    Lu, B., Dong, L., Yi, D., Zhang, M., Zhu, C., Li, X., Yi, C., 2020. Transposase-assisted tagmentation of RNA/DNA hybrid duplexes. Elife 9, e54919.
    McEvoy, C.R., Semple, T., Yellapu, B., Choong, D.Y., Xu, H., Mir Arnau, G., Fellowes, A.P., Fox, S.B., 2020. Improved next-generation sequencing precapture library yields and sequencing parameters using on-bead PCR. Biotechniques 68, 48-51.
    Meyer, K.D., Saletore, Y., Zumbo, P., Elemento, O., Mason, C.E., Jaffrey, S.R., 2012. Comprehensive analysis of mRNA methylation reveals enrichment in 3' UTRs and near stop codons. Cell 149, 1635-1646.
    Niu, Y., Lin, Z., Wan, A., Chen, H., Liang, H., Sun, L., Wang, Y., Li, X., Xiong, X.F., Wei, B., et al., 2019. RNA N6-methyladenosine demethylase FTO promotes breast tumor progression through inhibiting BNIP3. Mol. Cancer 18, 46.
    Tuncel, G., Kalkan, R., 2019. Importance of m N6-methyladenosine (m6A) RNA modification in cancer. Med. Oncol. 36, 36.
    Vu, L.P., Pickering, B.F., Cheng, Y., Zaccara, S., Nguyen, D., Minuesa, G., Chou, T., Chow, A., Saletore, Y., MacKay, M., et al., 2017. The N6-methyladenosine (m6A)-forming enzyme METTL3 controls myeloid differentiation of normal hematopoietic and leukemia cells. Nat. Med. 23, 1369-1376.
    Zeng, Y., Wang, S., Gao, S., Soares, F., Ahmed, M., Guo, H., Wang, M., Hua, J.T., Guan, J., Moran, M.F., et al., 2018. Refined RIP-seq protocol for epitranscriptome analysis with low input materials. PLoS Biol. 16, e2006092.
    Zhao, W., Qi, X., Liu, L., Ma, S., Liu, J., Wu, J., 2020. Epigenetic regulation of m6A modifications in human cancer. Mol. Ther. Nucleic Acids 19, 405-412.
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (600) PDF downloads (117) Cited by ()
    Proportional views
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return