Improving the Cpf1-mediated base editing system by combining dCas9/dead sgRNA with human APOBEC3A variants

Meng Lian; Fangbing Chen; Xingyun Huang; Xiaozhu Zhao; Shixue Gou; Nan Li; Qin Jin; Hui Shi; Yanhui Liang; Jingke Xie; Weikai Ge; Zhenpeng Zhuang; Jiaowei Wang; Yinghua Ye; Yi Yang; Kepin Wang; Liangxue Lai; Han Wu

doi:10.1016/j.jgg.2020.07.010

Volume 48 Issue 1

Jan. 2021

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Improving the Cpf1-mediated base editing system by combining dCas9/dead sgRNA with human APOBEC3A variants

doi: 10.1016/j.jgg.2020.07.010

Meng Lian^{1, 2, 1},
Fangbing Chen^{3, 4, 1},
Xingyun Huang^{3, 4, 1},
Xiaozhu Zhao^{3, 4},
Shixue Gou^{3, 4},
Nan Li^{3, 4},
Qin Jin^{3, 4},
Hui Shi^{3, 4},
Yanhui Liang^{3, 4},
Jingke Xie^{3, 4},
Weikai Ge^{3, 4},
Zhenpeng Zhuang^{3, 4},
Jiaowei Wang^{3, 4},
Yinghua Ye^{5, 6, 7},
Yi Yang⁸,
Kepin Wang^{9, 10, 11},
Liangxue Lai^{9, 10, 11},
Han Wu^{9, 10, 11}

1
CAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China
2
Institute of Physical Science and Information Technology, Anhui University, Hefei 230601, China
3
CAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China
4
University of Chinese Academy of Sciences, Beijing 100049, China
5
CAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China
6
Research Unit of Generation of Large Animal Disease Models, Chinese Academy of Medical Sciences (2019RU015), Guangzhou 510530, China
7
Bioland Laboratory (Guangzhou Regenerative Medicine and Health Guangdong Laboratory), Guangzhou 510005, China
8
Key Laboratory for Major Obstetrics Diseases of Guangdong Province, Key Laboratory of Reproduction and Genetics of Guangdong Higher Education Institutes, The Third Hospital Affiliated of Guangzhou Medical University, Guangzhou 510150, China
9
CAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China
10
Research Unit of Generation of Large Animal Disease Models, Chinese Academy of Medical Sciences (2019RU015), Guangzhou 510530, China
11
Bioland Laboratory (Guangzhou Regenerative Medicine and Health Guangdong Laboratory), Guangzhou 510005, China

More Information

Corresponding author: E-mail address: wang_kepin@gibh.ac.cn (Kepin Wang); E-mail address: lai_liangxue@gibh.ac.cn (Liangxue Lai); E-mail address: wu_han@gibh.ac.cn (Han Wu)
Publish Date: 2021-01-20

Abstract

These authors contributed equally to this work.

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References(19)

References

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