Cost-effective generation of A-to-G mutant mice by zygote electroporation of adenine base editor ribonucleoproteins

Hongwei Sun; Shengyao Zhi; Guifang Wu; Guanglan Wu; Tianqi Cao; Hu Hao; Zhou Songyang; Puping Liang; Junjiu Huang

doi:10.1016/j.jgg.2020.05.005

Volume 47 Issue 6

Jun. 2020

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Article Navigation > Journal of Genetics and Genomics > 2020 > 47(6): 337-340

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Cost-effective generation of A-to-G mutant mice by zygote electroporation of adenine base editor ribonucleoproteins

doi: 10.1016/j.jgg.2020.05.005

Hongwei Sun^{1, 1},
Shengyao Zhi^{1, 1},
Guifang Wu¹,
Guanglan Wu¹,
Tianqi Cao¹,
Hu Hao²,
Zhou Songyang^{3, 4, 5, 6},
Puping Liang^{7, 8},
Junjiu Huang^{9, 10, 11}

1
MOE Key Laboratory of Gene Function and Regulation and Guangzhou Key Laboratory of Healthy Aging Research, SYSU-BCM Joint Research Center, School of Life Sciences, Sun Yat-sen University, Guangzhou, 510275, China
2
Department of Paediatrics, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510655, China
3
MOE Key Laboratory of Gene Function and Regulation and Guangzhou Key Laboratory of Healthy Aging Research, SYSU-BCM Joint Research Center, School of Life Sciences, Sun Yat-sen University, Guangzhou, 510275, China
4
Key Laboratory of Reproductive Medicine of Guangdong Province, School of Life Sciences, and the First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510275, China
5
State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, 510060, China
6
Verna and Marrs Mclean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, TX, 77030, USA
7
MOE Key Laboratory of Gene Function and Regulation and Guangzhou Key Laboratory of Healthy Aging Research, SYSU-BCM Joint Research Center, School of Life Sciences, Sun Yat-sen University, Guangzhou, 510275, China
8
Key Laboratory of Reproductive Medicine of Guangdong Province, School of Life Sciences, and the First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510275, China
9
MOE Key Laboratory of Gene Function and Regulation and Guangzhou Key Laboratory of Healthy Aging Research, SYSU-BCM Joint Research Center, School of Life Sciences, Sun Yat-sen University, Guangzhou, 510275, China
10
Key Laboratory of Reproductive Medicine of Guangdong Province, School of Life Sciences, and the First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510275, China
11
State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, 510060, China

More Information

Corresponding author: E-mail address: songyanz@mail.sysu.edu.cn (Zhou Songyang); E-mail address: liangpp5@mail.sysu.edu.cn (Puping Liang); E-mail address: hjunjiu@mail.sysu.edu.cn (Junjiu Huang)
Publish Date: 2020-06-25

Abstract

These authors contributed equally to this work.

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References(29)

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