Site-directed RNA editing (SDRE): Off-target effects and their countermeasures

Shaoshuai Mao; Yajing Liu; Shisheng Huang; Xingxu Huang; Tian Chi

doi:10.1016/j.jgg.2019.11.005

Volume 46 Issue 11

Nov. 2019

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Article Navigation > Journal of Genetics and Genomics > 2019 > 46(11): 531-535

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Site-directed RNA editing (SDRE): Off-target effects and their countermeasures

doi: 10.1016/j.jgg.2019.11.005

Shaoshuai Mao^{a, b, c},
Yajing Liu^{a, b, c},
Shisheng Huang^{a, b, c},
Xingxu Huang^a,
Tian Chi^{a, d}

a
School of Life Sciences and Technology, ShanghaiTech University, Shanghai, 201210, China
b
CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai, 200031, China
c
University of Chinese Academy of Sciences, Beijing, 100049, China
d
Department of Immunobiology, Yale University School of Medicine, New Haven, CT 06520, USA

More Information

Corresponding author: E-mail address: chitian@shanghaitech.edu.cn (Tian Chi)
Received Date: 2019-07-18
Accepted Date: 2019-11-04
Rev Recd Date: 2019-10-08

Available Online: 2019-11-27

Publish Date: 2019-11-20

Abstract

Abstract

Site-directed RNA editing (SDRE) is invaluable to basic research and clinical applications and has emerged as a new frontier in genome editing. The past few years have witnessed a surge of interest in SDRE, with SDRE tools emerging at a breathtaking pace. However, off-target effects of SDRE remain a tough problem, which constitutes a major hurdle to their clinical applications. Here we discuss the diverse strategies for combating off-target editing, drawing lessons from the published studies as well as our ongoing research. Overall, SDRE is still at its infancy, with significant challenges and exciting opportunities ahead.
- Gene editing,
- RNA editing,
- Cas13,
- Off-target,
- Specificity

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References(28)

References

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