A highly efficient in vivo plasmid editing tool based on CRISPR-Cas12a and phage λ Red recombineering

Yiman Geng; Haiqin Yan; Pei Li; Gaixian Ren; Xiaopeng Guo; Peiqi Yin; Leiliang Zhang; Zhaohui Qian; Zhendong Zhao; Yi-Cheng Sun

doi:10.1016/j.jgg.2019.07.006

Volume 46 Issue 9

Sep. 2019

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Article Navigation > Journal of Genetics and Genomics > 2019 > 46(9): 455-458

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A highly efficient in vivo plasmid editing tool based on CRISPR-Cas12a and phage λ Red recombineering

doi: 10.1016/j.jgg.2019.07.006

1
NHC Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Center for Tuberculosis Research, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100730, China
2
Department of Basic Medical Sciences, Anhui Key Laboratory of Infection and Immunity, Bengbu Medical College, Bengbu, 233030, China
3
NHC Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Center for Tuberculosis Research, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100730, China

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Corresponding author: E-mail address: zqian2013@sina.com (Zhaohui Qian); E-mail address: timjszzd@163.com (Zhendong Zhao); E-mail address: sunyc@ipbcams.ac.cn (Yi-Cheng Sun)
Received Date: 2019-03-10
Accepted Date: 2019-07-04
Rev Recd Date: 2019-06-16

Available Online: 2019-09-10

Publish Date: 2019-09-20

Abstract

These authors contributed equally to this work.

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References(17)

References

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