Identification and characterization of rice blast resistance gene Pid4 by a combination of transcriptomic profiling and genome analysis

Zhixiong Chen; Wen Zhao; Xiaobo Zhu; Chengdong Zou; Junjie Yin; Mawsheng Chern; Xiaogang Zhou; Heng Ying; Xin Jiang; Yongzhen Li; Haicheng Liao; Mengping Cheng; Weitao Li; Min He; Jing Wang; Jichun Wang; Bingtian Ma; Jirui Wang; Shigui Li; Lihuang Zhu; Xuewei Chen

doi:10.1016/j.jgg.2018.10.007

Volume 45 Issue 12

Dec. 2018

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Identification and characterization of rice blast resistance gene Pid4 by a combination of transcriptomic profiling and genome analysis

doi: 10.1016/j.jgg.2018.10.007

a
State Key Laboratory of Crop Genetics of Disease Resistance and Disease Control, State Key Laboratory of Hybrid Rice, Key Laboratory of Major Crop Diseases & Collaborative Innovation Center for Hybrid Rice in Yangtze River Basin, Rice Research Institute, Sichuan Agricultural University, Sichuan 611130, China
b
Department of Plant Pathology, University of California, Davis, CA 95616, USA
c
Triticeae Research Institute, Sichuan Agricultural University, Sichuan 611130, China
d
State Key Laboratory of Plant Genomics, National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China

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Corresponding author: E-mail address: lhzhu@genetics.ac.cn (Lihuang Zhu); E-mail address: xwchen@sicau.edu.cn (Xuewei Chen)
Received Date: 2018-06-08
Accepted Date: 2018-10-18
Rev Recd Date: 2018-09-11

Available Online: 2018-12-31

Publish Date: 2018-12-20

Abstract

Abstract

Map-based cloning of plant disease resistance (R) genes is time-consuming. Here, we reported the isolation of blast R gene Pid4 using comparative transcriptomic profiling and genome-wide sequence analysis. Pid4 encodes a coiled-coil nucleotide-binding site leucine-rich repeat (CC-NBS-LRR) protein and is constitutively expressed at diverse developmental stages in the rice variety Digu. The Pid4 protein is localized in both the nucleus and cytoplasm. Introduction of Pid4 into susceptible rice cultivars confers race-specific resistance to leaf and neck blast. Amino acid sequence comparison and blast resistance spectrum tests showed that Pid4 is a novel R gene, different from the previously reported R genes located in the same gene cluster. A Pid4 Indel marker was developed to facilitate the identification of Pid4 in different rice varieties. We demonstrated that a plant R gene can be quickly isolated using transcriptomic profiling coupled with genome-wide sequence analysis.
- Blast disease,
- Genome-wide sequence analysis,
- Resistance,
- Rice,
- Transcriptome

These authors contributed equally to this work.

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References(62)

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