Efficient generation of pink-fruited tomatoes using CRISPR/Cas9 system

Lei Deng; Hang Wang; Chuanlong Sun; Qian Li; Hongling Jiang; Minmin Du; Chang-Bao Li; Chuanyou Li

doi:10.1016/j.jgg.2017.10.002

Volume 45 Issue 1

Jan. 2018

Turn off MathJax

Article Contents

Article Navigation > Journal of Genetics and Genomics > 2018 > 45(1): 51-54

PDF( 1446 KB)

Efficient generation of pink-fruited tomatoes using CRISPR/Cas9 system

doi: 10.1016/j.jgg.2017.10.002

1
State Key Laboratory of Plant Genomics, National Centre for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
2
State Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai'an 271018, China
3
State Key Laboratory of Plant Genomics, National Centre for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
4
Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Ministry of Agriculture, Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China
5
State Key Laboratory of Plant Genomics, National Centre for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
6
State Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai'an 271018, China

More Information

Corresponding author: E-mail address: lichangbao@nercv.org (Chang-Bao Li); E-mail address: cyli@genetics.ac.cn (Chuanyou Li)
Received Date: 2017-10-17
Accepted Date: 2017-10-31
Rev Recd Date: 2017-10-31

Available Online: 2017-11-06

Publish Date: 2018-01-20

Abstract

These authors contributed equally to this work.

FullText(HTML)

References(20)

References

[1]	Adato, A., Mandel, T., Mintz-Oron, S. et al. PLoS Genet., 5 (2009),p. e1000777
[2]	Ballester, A.R., Molthoff, J., de Vos, R. et al. Biochemical and molecular analysis of pink tomatoes: deregulated expression of the gene encoding transcription factor S1MYB12 leads to pink tomato fruit color Plant Physiol., 152 (2010),pp. 71-84
[3]	Brooks, C., Nekrasov, V., Lippman, Z.B. et al. Efficient gene editing in tomato in the first generation using the clustered regularly interspaced short palindromic repeats/CRISPR-associated9 system Plant Physiol., 166 (2014),pp. 1292-1297
[4]	Cong, L., Ran, F.A., Cox, D. et al. Multiplex genome engineering using CRISPR/Cas systems Science, 339 (2013),pp. 819-823
[5]	Du, M.M., Zhao, J.H., Tzeng, D.T.W. et al. MYC2 orchestrates a hierarchical transcriptional cascade that regulates jasmonate-mediated plant immunity in tomato Plant Cell, 29 (2017),pp. 1883-1906
[6]	Feng, Z.Y., Zhang, B.T., Ding, W.N. et al. Efficient genome editing in plants using a CRISPR/Cas system Cell Res., 23 (2013),pp. 1229-1232
[7]	Fernandez-Moreno, J.P., Tzfadia, O., Forment, J. et al. Characterization of a new pink-fruited tomato mutant results in the identification of a null allele of the SlMYB12 transcription factor Plant Physiol., 171 (2016),pp. 1821-1836
[8]	Gao, X., Chen, J., Dai, X. et al. Plant Physiol., 171 (2016),pp. 1794-1800
[9]	He, Y., Zhang, T., Yang, N. et al. Self-cleaving ribozymes enable the production of guide RNAs from unlimited choices of promoters for CRISPR/Cas9 mediated genome editing J. Genet. Genomics, 44 (2017),pp. 469-472
[10]	Jiang, W., Zhou, H., Bi, H. et al. Nucleic Acids Res., 41 (2013),p. e188
[11]	Li, J., Sun, Y., Du, J. et al. Generation of targeted point mutations in rice by a modified CRISPR/Cas9 system Mol. Plant, 10 (2017),pp. 526-529
[12]	Li, J., Zhang, H., Si, X. et al. J. Genet. Genomics, 44 (2017),pp. 465-468
[13]	Lin, T., Zhu, G., Zhang, J. et al. Genomic analyses provide insights into the history of tomato breeding Nat. Genet., 46 (2014),pp. 1220-1226
[14]	Miao, J., Guo, D.S., Zhang, J.Z. et al. Targeted mutagenesis in rice using CRISPR-Cas system Cell Res., 23 (2013),pp. 1233-1236
[15]	Shan, Q.W., Wang, Y.P., Li, J. et al. Targeted genome modification of crop plants using a CRISPR-Cas system Nat. Biotechnol., 31 (2013),pp. 686-688
[16]	The Tomato Genome Consortium The tomato genome sequence provides insights into fleshy fruit evolution Nature, 485 (2012),pp. 635-641
[17]	Wang, Y., Cheng, X., Shan, Q. et al. Simultaneous editing of three homoeoalleles in hexaploid bread wheat confers heritable resistance to powdery mildew Nat. Biotechnol., 32 (2014),pp. 947-951
[18]	Wang, Z.P., Xing, H.L., Dong, L. et al. Genome Biol., 16 (2015),p. 144
[19]	Xie, K., Yang, Y. RNA-guided genome editing in plants using a CRISPR-Cas system Mol. Plant, 6 (2013),pp. 1975-1983
[20]	Zhang, H., Zhang, J., Wei, P. et al. The CRISPR/Cas9 system produces specific and homozygous targeted gene editing in rice in one generation Plant Biotechnol. J., 12 (2014),pp. 797-807