Essential roles of stat5.1/stat5b in controlling fish somatic growth

Shuting Xiong; Jie Mei; Peipei Huang; Jing Jing; Zhi Li; Jingliang Kang; Jian-Fang Gui

doi:10.1016/j.jgg.2017.07.006

Volume 44 Issue 12

Dec. 2017

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Essential roles of stat5.1/stat5b in controlling fish somatic growth

doi: 10.1016/j.jgg.2017.07.006

a
College of Fisheries, Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070, China
b
State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, University of the Chinese Academy of Sciences, Wuhan 430072, China
c
Medical Research Institute, Wuhan University, Wuhan 430071, China

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Corresponding author: E-mail address: jmei@mail.hzau.edu.cn (Jie Mei); E-mail address: jfgui@ihb.ac.cn (Jian-Fang Gui)
Received Date: 2017-05-05
Accepted Date: 2017-07-19
Rev Recd Date: 2017-06-21

Available Online: 2017-11-16

Publish Date: 2017-12-20

Abstract

Abstract

Signal transducer and activator of transcription 5b (STAT5b) has been identified as a key downstream mediator of growth hormone (GH) signaling in somatic growth of mammalian. However, the corresponding homologue gene of Stat5b is unknown in fish species. In this study, we generated loss-of-function mutants in stat5.1 and stat5.2, two stat5 homologues existing in zebrafish. In stat5.1-deficient zebrafish, a significant reduction of body length and body weight was detected in the embryos/larvae and adults compared with the wild-type control fish, and sexual size dimorphism in adult zebrafish was also eliminated. However, the stat5.2-deficient zebrafish displayed a normal developmental phenotype during all lifespan. Chromatin immunoprecipitation combined with deep sequencing (ChIP-seq) method was adopted to further investigate the potential transcriptional targets of Stat5 protein and cast much light upon the biological function of Stat5. We identified more than 800 genes as transcriptional targets of Stat5 during zebrafish embryogenesis. KEGG analysis indicated that the Stat5 target gene network is predominantly linked to the metabolic pathways, neuroactive ligand-receptor interaction and JAK-STAT signaling pathways. Further validation studies suggested that Stat5.1 protein could directly regulate the expression of gh1, and stat5.1-mutated zebrafish showed a reduction of gh1 mRNA level. In the present study, stat5.1 was revealed as the corresponding homologue gene of Stat5b in fish species. Additionally, we found a novel molecular interaction between Stat5.1/Stat5b and GH, and unraveled a positive feedback loop Stat5.1-GH-Stat5.1 which is necessary for somatic growth and body development in zebrafish.
- Zebrafish,
- Stat5,
- Body growth,
- Sexual size dimorphism,
- Growth hormone

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References(45)

References

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