LMM5.1 and LMM5.4, two eukaryotic translation elongation factor 1A-like gene family members, negatively affect cell death and disease resistance in rice

Jiying Zhao; Pengcheng Liu; Chunrong Li; Yanyan Wang; Lequn Guo; Guanghuai Jiang; Wenxue Zhai

doi:10.1016/j.jgg.2016.12.005

Volume 44 Issue 2

Feb. 2017

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LMM5.1 and LMM5.4, two eukaryotic translation elongation factor 1A-like gene family members, negatively affect cell death and disease resistance in rice

doi: 10.1016/j.jgg.2016.12.005

a
Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
b
Kaifeng Institute for Food and Drug Control, Kaifeng 475000, China

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Corresponding author: E-mail address: ghjiang@genetics.ac.cn (Guanghuai Jiang); E-mail address: wxzhai@genetics.ac.cn (Wenxue Zhai)
Received Date: 2016-11-15
Accepted Date: 2016-12-26
Rev Recd Date: 2016-12-22

Available Online: 2016-12-27

Publish Date: 2017-02-20

Abstract

Abstract

Lesion mimic mutant (LMM) genes, stimulating lesion formation in the absence of pathogens, play significant roles in immune response. In this study, we characterized a rice lesion mimic mutant, lmm5, which displayed light-dependent spontaneous lesions. Additionally, lmm5 plants exhibited enhanced resistance to all of the tested races of Magnaporthe oryzae and Xanthomonas oryzae pv. oryzae (Xoo) by increasing the expression of defense-related genes and the accumulation of hydrogen peroxide. Genetic analysis showed that the lesion mimic phenotype of lmm5 was controlled by two genes, lmm5.1 and lmm5.4, which were isolated with a map-based cloning strategy. Remarkably, LMM5.1 and LMM5.4 share a 97.4% amino acid sequence identity, and they each encode a eukaryotic translation elongation factor 1A (eEF1A)-like protein. Besides, LMM5.1 and LMM5.4 were expressed in a tissue-specific and an indica-specific manner, respectively. In addition, high-throughput mRNA sequencing analysis confirmed that the basal immunity was constitutively activated in the lmm5 mutant. Taken together, these results suggest that the homologous eEF1A-like genes, LMM5.1 and LMM5.4, negatively affect cell death and disease resistance in rice.
- Lesion mimic,
- eEF1A-like gene,
- Cell death,
- Disease resistance,
- Rice

These authors contributed equally to this work.

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