miR-503-3p promotes epithelial–mesenchymal transition in breast cancer by directly targeting SMAD2 and E-cadherin

Zitong Zhao; Xinyi Fan; Lanfang Jiang; Zhongqiu Xu; Liyan Xue; Qimin Zhan; Yongmei Song

doi:10.1016/j.jgg.2016.10.005

Volume 44 Issue 2

Feb. 2017

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Article Navigation > Journal of Genetics and Genomics > 2017 > 44(2): 75-84

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miR-503-3p promotes epithelial–mesenchymal transition in breast cancer by directly targeting SMAD2 and E-cadherin

doi: 10.1016/j.jgg.2016.10.005

a
State Key Laboratory of Molecular Oncology, National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China
b
Qiqihar Medical University, Qiqihar 161000, China

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Corresponding author: E-mail address: songym@cicams.ac.cn (Yongmei Song)
Received Date: 2016-03-10
Accepted Date: 2016-10-28
Rev Recd Date: 2016-10-21

Available Online: 2016-11-01

Publish Date: 2017-02-20

Abstract

Abstract

Although progress in clinical and basic research has significantly increased our understanding of breast cancer, little is known about the molecular mechanism underlying breast cancer metastasis. Identification of effective therapeutic targets to prevent breast cancer metastasis is urgently needed. The function of miR-503-3p has been investigated in other cancers, but its role in breast cancer remains undefined. Here, we found that miR-503-3p was overexpressed in breast cancer tissue and plasma compared with adjacent normal breast tissue and with plasma from healthy individuals. Moreover, we identified miR-503-3p to be an oncogene of breast cancer cell proliferation, migration and invasion. Upregulation of miR-503-3p in breast cancer cells inhibited expression of epithelial–mesenchymal transition (EMT)-related protein SMAD2 and the epithelial marker protein E-cadherin by directly binding to their mRNA 3′ untranslated region, whereas increased expression of mesenchymal marker proteins, including vimentin and N-cadherin. Taken together, our findings support a critical role for miR-503-3p in induction of breast cancer EMT and suggest that plasma miR-503-3p may be a useful diagnostic biomarker for breast cancer.
- Breast cancer,
- miR-503-3p,
- Plasma miRNA,
- SMAD2,
- E-cadherin

These authors contributed equally to this work.

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References(23)

References

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