A functional centromere lacking CentO sequences in a newly formed ring chromosome in rice

Rui Yang; Yafei Li; Yan Su; Yi Shen; Ding Tang; Qiong Luo; Zhukuan Cheng

doi:10.1016/j.jgg.2016.09.006

Volume 43 Issue 12

Dec. 2016

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Article Navigation > Journal of Genetics and Genomics > 2016 > 43(12): 694-701

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A functional centromere lacking CentO sequences in a newly formed ring chromosome in rice

doi: 10.1016/j.jgg.2016.09.006

Rui Yang^{a, #},
Yafei Li^{b, #},
Yan Su^b,
Yi Shen^b,
Ding Tang^b,
Qiong Luo^a,
Zhukuan Cheng^b

a
State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Ministry of Education Key Laboratory of Agriculture Biodiversity for Plant Disease Management, Yunnan Agricultural University, Kunming 650201, China
b
State Key Laboratory of Plant Genomics, Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China

More Information

Corresponding author: E-mail address: qiongbf@aliyun.com (Qiong Luo); E-mail address: zkcheng@genetics.ac.cn (Zhukuan Cheng)
Received Date: 2016-07-03
Accepted Date: 2016-09-22
Rev Recd Date: 2016-09-10

Available Online: 2016-10-13

Publish Date: 2016-12-20

Abstract

Abstract

An awned rice (Oryza sativa) plant carrying a tiny extra chromosome was discovered among the progeny of a telotrisomic line 2n+4L. Fluorescence in situ hybridization (FISH) using chromosome specific BAC clones revealed that this extra chromosome was a ring chromosome derived from part of the long arm of chromosome 4. So the aneuploidy plant was accordingly named as 2n+4L ring. We did not detect any CentO FISH signals on the ring chromosome, and found only the centromeric probe Centromeric Retrotransposon of Rice (CRR) was co-localized with the centromere-specific histone CENH3 as revealed by sequential FISH after immunodetection. The extra ring chromosome exhibited a unique segregation pattern during meiosis, including no pairing between the ring chromosome and normal chromosome 4 during prophase I and pre-separation of sister chromatids at anaphase I.
- Rice,
- Ring chromosome,
- Meiosis,
- Centromere

These authors contributed equally to this work.

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References(50)

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