Construction and Validation of a Dual-Transgene Vector System for Stable Transformation in Plants

Zhimin He; Bin Liu; Xu Wang; Mingdi Bian; Reqing He; Jindong Yan; Ming Zhong; Xiaoying Zhao; Xuanming Liu

doi:10.1016/j.jgg.2016.02.005

Volume 43 Issue 4

Apr. 2016

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Article Navigation > Journal of Genetics and Genomics > 2016 > 43(4): 207-215

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Construction and Validation of a Dual-Transgene Vector System for Stable Transformation in Plants

doi: 10.1016/j.jgg.2016.02.005

a
Hunan Province Key Laboratory of Plant Functional Genomics and Developmental Regulation, College of Biology, Hunan University, Changsha 410082, China
b
Department of Molecular, Cell & Developmental Biology, University of California, Los Angeles, CA 90095, USA
c
Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
d
College of Plant Sciences, Jilin University, Changchun 130062, China
e
State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha 410082, China

More Information

Corresponding author: E-mail address: xiaoyzhao@hnu.edu.cn (Xiaoying Zhao); E-mail address: xmL05@hnu.edu.cn (Xuanming Liu)
Received Date: 2015-07-05
Accepted Date: 2016-02-25
Rev Recd Date: 2016-02-14

Available Online: 2016-03-10

Publish Date: 2016-04-20

Abstract

Abstract

In this study, we constructed dual-transgene vectors (pDT1, pDT7, and pDT7G) that simultaneously co-expressed two genes in plants. ACTIN2 and UBQ10 promoters were used to control the expression of these two genes. The 4×Myc, 3×HA, and 3×Flag reporter genes allowed for the convenient identification of a tunable co-expression system in plants, whereas the dexamethasone (Dex) inducible reporter gene C-terminus of the glucocorticoid receptor (cGR) provided Dex-dependent translocation of the fusion gene between the nucleus and cytoplasm. The function of pDT vectors was validated using four pairwise genes in Nicotiana benthamiana or Arabidopsis thaliana. The co-expression efficiency of two genes from the pDT1 and pDT7G vectors was 35% and 42%, respectively, which ensured the generation of sufficient transgenic materials. These pDT vectors are simple, reliable, efficient, and time-saving tools for the co-expression of two genes through a single transformation event and can be used in the study of protein–protein interactions or multi-component complexes.
- Co-expression,
- Dual-transgene vector,
- pDT1,
- pDT7,
- pDT7G

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References(29)

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