Identification of microRNAs in rice root in response to nitrate and ammonium

Hua Li; Bin Hu; Wei Wang; Zhihua Zhang; Yan Liang; Xiaokai Gao; Peng Li; Yongqiang Liu; Lianhe Zhang; Chengcai Chu

doi:10.1016/j.jgg.2015.12.002

Volume 43 Issue 11

Nov. 2016

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Identification of microRNAs in rice root in response to nitrate and ammonium

doi: 10.1016/j.jgg.2015.12.002

Hua Li^{a, b},
Bin Hu^a,
Wei Wang^a,
Zhihua Zhang^{a, b},
Yan Liang^{a, b},
Xiaokai Gao^c,
Peng Li^c,
Yongqiang Liu^{a, b},
Lianhe Zhang^c,
Chengcai Chu^a

a
State Key Laboratory of Plant Genomics, National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
b
College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China
c
School of Agriculture, Henan University of Science and Technology, Luoyang 471023, China

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Corresponding author: E-mail address: ccchu@genetics.ac.cn (Chengcai Chu)
Received Date: 2015-10-21
Accepted Date: 2015-12-18
Rev Recd Date: 2015-12-15

Available Online: 2016-05-24

Publish Date: 2016-11-20

Abstract

Abstract

Nitrate and ammonium are two major nitrogen (N) sources for higher plants, but they differ in utilization and signaling. MicroRNAs (miRNAs) play an essential role in N signal transduction; however, knowledge remains limited about the regulatory role of miRNAs responsive to different N sources, especially in crop plants. To get global overview on miRNAs involved in N response in rice, we performed high-throughput small RNA-sequencing under different nitrate and ammonium treatments. The results demonstrated that only 16 and 11 miRNAs were significantly induced by nitrate and ammonium under short-term treatment, respectively. However, 60 differentially expressed miRNAs were found between nitrate and ammonium under long-term cultivation. These results suggested that miRNA response greatly differentiates between nitrate and ammonium treatments. Furthermore, 44 miRNAs were found to be differentially expressed between high- and low-N conditions. Our study reveals comprehensive expression profiling of miRNAs responsive to different N sources and different N treatments, which advances our understanding on the regulation of different N signaling and homeostasis mediated by miRNAs.
- Rice,
- MicroRNA,
- Nitrogen,
- Nitrate,
- Ammonium

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References(77)

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