Involvement of a Putative Bipartite Transit Peptide in Targeting Rice Pheophorbide a Oxygenase into Chloroplasts for Chlorophyll Degradation during Leaf Senescence

Qingjun Xie; Yan Liang; Jian Zhang; Huakun Zheng; Guojun Dong; Qian Qian; Jianru Zuo

doi:10.1016/j.jgg.2015.09.012

Volume 43 Issue 3

Mar. 2016

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Article Navigation > Journal of Genetics and Genomics > 2016 > 43(3): 145-154

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Involvement of a Putative Bipartite Transit Peptide in Targeting Rice Pheophorbide a Oxygenase into Chloroplasts for Chlorophyll Degradation during Leaf Senescence

doi: 10.1016/j.jgg.2015.09.012

Qingjun Xie^{a, b, c, #},
Yan Liang^{a, #},
Jian Zhang^a,
Huakun Zheng^{a, b},
Guojun Dong^d,
Qian Qian^d,
Jianru Zuo^a

a
State Key Laboratory of Plant Genomics and National Plant Gene Research Center, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
b
The University of Chinese Academy of Sciences, Beijing 100049, China
c
State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, China
d
State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310006, China

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Corresponding author: E-mail address: qianqian188@hotmail.com (Qian Qian); E-mail address: jrzuo@genetics.ac.cn (Jianru Zuo)
Received Date: 2015-07-08
Accepted Date: 2015-09-15
Rev Recd Date: 2015-08-27

Available Online: 2016-01-08

Publish Date: 2016-03-20

Abstract

Abstract

Leaf senescence is one of the major factors contributing to the productivity and the grain quality in crops. The regulatory mechanism of leaf senescence remains largely unknown. Here, we report the identification and characterization of a rice (eas1) mutant, which displayed an early leaf senescence phenotype, accompanying by dwarfism and reduced tiller number, eventually leading to the reduction of grain yield. Map-based cloning revealed that the nuclear gene EAS1 encodes a pheophorbide a oxygenase (PaO), a key enzyme for chlorophyll breakdown. A highly conserved Thr residue of PaO was mutated into Ile in the eas1 mutant. Phylogenetic analysis indicates that PaO is an evolutionarily conserved protein, and EAS1 is 68% identical to the Arabidopsis ACCERLERATED CELL DEATH (ACD1) protein. Unlike ACD1 that contains a single transit peptide, EAS1 contains two putative transit peptides at its N-terminus, which are essential for its functionality, suggesting that targeting of EAS1 to the chloroplast is likely mediated by a putative bipartite transit peptide. Consistently, only a short version of EAS1 lacking the first putative transit peptide, but not the full-length EAS1, was capable of rescuing theArabidopsis acd1 mutant phenotype. These results suggest that rice EAS1 represents a functional PaO, which is involved in chlorophyll degradation and may utilize a unique mechanism for its import into the chloroplast.
- Targeting,
- Transit peptide,
- Leaf senescence,
- Rice

Present address: Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China.

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References(40)

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