SCAB3 Is Required for Reorganization of Actin Filaments during Light Quality Changes

Chongwu Wang; Yuan Zheng; Yang Zhao; Yi Zhao; Jigang Li; Yan Guo

doi:10.1016/j.jgg.2015.02.005

Volume 42 Issue 4

Apr. 2015

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Article Navigation > Journal of Genetics and Genomics > 2015 > 42(4): 161-168

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SCAB3 Is Required for Reorganization of Actin Filaments during Light Quality Changes

doi: 10.1016/j.jgg.2015.02.005

a
State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100193, China
b
School of Life Science & Technology, Nanyang Normal University, Nanyang 473061, China

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Corresponding author: E-mail address: guoyan@cau.edu.cn (Yan Guo)
Received Date: 2014-11-04
Accepted Date: 2015-02-13
Rev Recd Date: 2015-02-11

Available Online: 2015-02-23

Publish Date: 2015-04-20

Abstract

Abstract

The STOMATAL CLOSURE-RELATED ACTIN BINDING PROTEIN (SCAB) family is plant-specific, and its members all contain a novel actin binding domain. Here, we report that SCAB3, a homolog of SCAB1, binds, stabilizes and bundles actin filaments. The SCAB3 promoter contains a cis-element which could be bound by the FHY3/FAR1 transcription factors. Consistently, the expression of SCAB3 is induced when plants were transferred from white light to far red light (T-Far Red) conditions. The scab3 mutants show defects in the control of hypocotyl elongation under T-Far Red condition, which may result from an impaired reorganization of actin filaments. Together, our results suggest that SCAB3 plays an important role in plant growth under changes of light conditions possibly by regulating actin filament dynamics.
- Cytoskeleton,
- Actin,
- Far Red light

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References(27)

References

[1]	bin Yusof, M.T., Kershaw, M.J., Soanes, D.M. et al. PLoS One, 9 (2014),p. e99760
[2]	Casal, J.J., Candia, A.N., Sellaro, R. Light perception and signalling by phytochrome A J. Exp. Bot., 65 (2014),pp. 2835-2845
[3]	Era, A., Tominaga, M., Ebine, K. et al. Plant Cell Physiol., 50 (2009),pp. 1041-1048
[4]	Huang, X., Ouyang, X., Deng, X.W. Beyond repression of photomorphogenesis: role switching of COP/DET/FUS in light signaling Curr. Opin. Plant Biol., 21 (2014),pp. 96-103
[5]	Hudson, M.E., Lisch, D.R., Quail, P.H. Plant J., 34 (2003),pp. 453-471
[6]	Ichikawa, S., Yamada, N., Suetsugu, N. et al. Red light, Phot1 and JAC1 modulate Phot2-dependent reorganization of chloroplast actin filaments and chloroplast avoidance movement Plant Cell Physiol., 52 (2011),pp. 1422-1432
[7]	Iwabuchi, K., Takagi, S. Plant Signal. Behav., 5 (2010),pp. 1010-1013
[8]	Kadota, A., Yamada, N., Suetsugu, N. et al. Proc. Natl. Acad. Sci. USA, 106 (2009),pp. 13106-13111
[9]	Li, G., Siddiqui, H., Teng, Y. et al. Nat. Cell Biol., 13 (2011),pp. 616-622
[10]	Lin, R., Wang, H. Plant Physiol., 136 (2004),pp. 4010-4022
[11]	Lin, R., Ding, L., Casola, C. et al. Transposase-derived transcription factors regulate light signaling in Arabidopsis Science, 318 (2007),pp. 1302-1305
[12]	Nagatani, A., Reed, J.W., Chory, J. Plant Physiol., 102 (1993),pp. 269-277
[13]	Ouyang, X., Li, J., Li, G. et al. Plant Cell, 23 (2011),pp. 2514-2535
[14]	Possart, A., Fleck, C., Hiltbrunner, A. Shedding (far-red) light on phytochrome mechanisms and responses in land plants Plant Sci., 217–218 (2014),pp. 36-46
[15]	Riedl, J., Crevenna, A.H., Kessenbrock, K. et al. Lifeact: a versatile marker to visualize F-actin Nat. Methods, 5 (2008),pp. 605-607
[16]	Sakurai, N., Domoto, K., Takagi, S. Planta, 221 (2005),pp. 66-74
[17]	Sharrock, R.A., Quail, P.H. Genes Dev., 3 (1989),pp. 1745-1757
[18]	Snowman, B.N., Kovar, D.R., Shevchenko, G. et al. Signal-mediated depolymerization of actin in pollen during the self-incompatibility response Plant Cell, 14 (2002),pp. 2613-2626
[19]	Somers, D.E., Sharrock, R.A., Tepperman, J.M. et al. Plant Cell, 3 (1991),pp. 1263-1274
[20]	Vidali, L., Rounds, C.M., Hepler, P.K. et al. Lifeact-mEGFP reveals a dynamic apical F-actin network in tip growing plant cells PLoS One, 4 (2009),p. e5744
[21]	Wang, H., Deng, X.W. EMBO J., 21 (2002),pp. 1339-1349
[22]	Wen, F., Wang, J., Xing, D. Plant Cell Physiol., 53 (2012),pp. 1366-1379
[23]	Whippo, C.W., Khurana, P., Davis, P.A. et al. THRUMIN1 is a light-regulated actin-bundling protein involved in chloroplast motility Curr. Biol., 21 (2011),pp. 59-64
[24]	Yanovsky, M.J., Whitelam, G.C., Casal, J.J. Plant Physiol., 123 (2000),pp. 235-242
[25]	Zhang, W., Zhao, Y., Guo, Y. et al. Plant actin-binding protein SCAB1 is dimeric actin cross-linker with atypical pleckstrin homology domain J. Biol. Chem., 287 (2012),pp. 11981-11990
[26]	Zhao, Y., Zhao, S., Mao, T. et al. Plant Cell, 23 (2011),pp. 2314-2330
[27]	Zhao, J., Zhang, W., Zhao, Y. et al. Plant Cell, 19 (2007),pp. 3805-3818