TaSCL14, a Novel Wheat (Triticum aestivum L.) GRAS Gene, Regulates Plant Growth, Photosynthesis, Tolerance to Photooxidative Stress, and Senescence

Kunmei Chen; Hongwei Li; Yaofeng Chen; Qi Zheng; Bin Li; Zhensheng Li

doi:10.1016/j.jgg.2014.11.002

Volume 42 Issue 1

Jan. 2015

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TaSCL14, a Novel Wheat (Triticum aestivum L.) GRAS Gene, Regulates Plant Growth, Photosynthesis, Tolerance to Photooxidative Stress, and Senescence

doi: 10.1016/j.jgg.2014.11.002

a
College of Agronomy, Northwest A&F University, Yangling 712100, Shaanxi, China
b
State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China

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Corresponding author: E-mail address: hwli@genetics.ac.cn (Hongwei Li); E-mail address: chenyf3828@126.com (Yaofeng Chen)
Received Date: 2014-04-28
Accepted Date: 2014-11-02
Rev Recd Date: 2014-10-31

Available Online: 2014-11-20

Publish Date: 2015-01-20

Abstract

Abstract

Rates of photosynthesis, tolerance to photooxidative stress, and senescence are all important physiological factors that affect plant development and thus agricultural productivity. GRAS proteins play essential roles in plant growth and development as well as in plant responses to biotic and abiotic stresses. So far fewGRAS genes in wheat (Triticum aestivum L.) have been characterized. A previous transcriptome analysis indicated that the expression of a GRAS gene (TaSCL14) was induced by high-light stress in Xiaoyan 54 (XY54), a common wheat cultivar with strong tolerance to high-light stress. In this study, TaSCL14 gene was isolated from XY54 and mapped on chromosome 4A. TaSCL14 was expressed in various wheat organs, with high levels in stems and roots. Our results confirmed that TaSCL14 expression was indeed responsive to high-light stress. Barley stripe mosaic virus (BSMV)-based virus-induced gene silencing (VIGS) of TaSCL14 in wheat was performed to help characterize its potential functions. Silencing of TaSCL14 resulted in inhibited plant growth, decreased photosynthetic capacity, and reduced tolerance to photooxidative stress. In addition, silencing of TaSCL14 in wheat promoted leaf senescence induced by darkness. These results suggest that TaSCL14 may act as a multifunctional regulator involved in plant growth, photosynthesis, tolerance to photooxidative stress, and senescence.
- Photosynthesis,
- Photooxidative resistance,
- Senescence,
- BSMV–VIGS

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References(48)

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