SPOROCYTELESS Is a Novel Embryophyte-Specific Transcription Repressor that Interacts with TPL and TCP Proteins in Arabidopsis

Guang-Hui Chen; Jia-Ying Sun; Man Liu; Jie Liu; Wei-Cai Yang

doi:10.1016/j.jgg.2014.08.009

Volume 41 Issue 12

Dec. 2014

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SPOROCYTELESS Is a Novel Embryophyte-Specific Transcription Repressor that Interacts with TPL and TCP Proteins in Arabidopsis

doi: 10.1016/j.jgg.2014.08.009

a
State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
b
The University of Chinese Academy of Sciences, 19 Yuquan Road, Shijingshan District, Beijing 100049, China

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Corresponding author: E-mail address: wcyang@genetics.ac.cn (Wei-Cai Yang)
Received Date: 2014-05-14
Accepted Date: 2014-08-19
Rev Recd Date: 2014-08-17

Available Online: 2014-11-07

Publish Date: 2014-12-20

Abstract

Abstract

Germlines in plants are formed de novo during post-embryonic development, while little is known about the mechanism that controls this process. In Arabidopsis, the earliest gene controlling this process is SPOROCYTELESS (SPL). A decade ago, we showed that loss of SPL function abolished sporogenesis in both male and female organs of Arabidopsis. However, its function is unclear up to now. In this study, we showed that SPL belongs to a novel transcription repressor family specific in embryophyte, which consists of 173 members in the land plants so far. All of them contain a conserved SPL-motif in their N-terminal and an ethylene-responsive element binding factor-associated amphiphilic repression (EAR) motif in the C-terminal, therefore designated as SPL-like, EAR-containing proteins (SPEARs). Consistently, SPL acts as a transcriptional repressor in yeast and tobacco cells, and SPEAR proteins are able to form homodimer and/or heterodimer with each otherin vitro. Furthermore, SPEARs interact with the TOPLESS (TPL) co-repressors via the EAR motif and TCP family transcription factors in yeast cells. Together, we propose that SPL and SPEARs most likely belong to a novel transcription repressor family in land plants which may play a variety of developmental roles in plants.
- Germline specification,
- Sporogenesis,
- SPL,
- Transcription repressor

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