Development of T. aestivum L.–H. californicum Alien Chromosome Lines and Assignment of Homoeologous Groups of Hordeum californicum Chromosomes

Yuhui Fang; Jingya Yuan; Zhangjun Wang; Haiyan Wang; Jin Xiao; Zhixi Yang; Ruiqi Zhang; Zengjun Qi; Weigang Xu; Lin Hu; Xiu-E Wang

doi:10.1016/j.jgg.2014.06.004

Volume 41 Issue 8

Aug. 2014

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Development of T. aestivum L.–H. californicum Alien Chromosome Lines and Assignment of Homoeologous Groups of Hordeum californicum Chromosomes

doi: 10.1016/j.jgg.2014.06.004

a
State Key Laboratory of Crop Genetics and Germplasm Enhancement, Cytogenetics Institute, Nanjing Agricultural University, Nanjing 210095, China
b
Wheat Research Center, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China

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Corresponding author: E-mail address: xiuew@njau.edu.cn (Xiu-E Wang)
Received Date: 2014-01-20
Accepted Date: 2014-06-16
Rev Recd Date: 2014-06-04

Available Online: 2014-06-23

Publish Date: 2014-08-20

Abstract

Abstract

Hordeum californicum (2n = 2x = 14, HH) is resistant to several wheat diseases and tolerant to lower nitrogen. In this study, a molecular karyotype of H. californicum chromosomes in the Triticum aestivum L. cv. Chinese Spring (CS)–H. californicum amphidiploid (2n = 6x = 56, AABBDDHH) was established. By genomic in situ hybridization (GISH) and multicolor fluorescent in situ hybridization (FISH) using repetitive DNA clones (pTa71, pTa794 and pSc119.2) as probes, the H. californicum chromosomes could be differentiated from each other and from the wheat chromosomes unequivocally. Based on molecular karyotype and marker analyses, 12 wheat–alien chromosome lines, including four disomic addition lines (DAH1, DAH3, DAH5 and DAH6), five telosomic addition lines (MtH7L, MtH1S, MtH1L, DtH6S and DtH6L), one multiple addition line involvingH. californicum chromosome H2, one disomic substitution line (DSH4) and one translocation line (TH7S/1BL), were identified from the progenies derived from the crosses of CS–H. californicum amphidiploid with common wheat varieties. A total of 482 EST (expressed sequence tag) or SSR (simple sequence repeat) markers specific for individual H. californicum chromosomes were identified, and 47, 50, 45, 49, 21, 51 and 40 markers were assigned to chromosomes H1, H2, H3, H4, H5, H6 and H7, respectively. According to the chromosome allocation of these markers, chromosomes H2, H3, H4, H5, and H7 of H. californicum have relationship with wheat homoeologous groups 5, 2, 6, 3, and 1, and hence could be designated as 5H^c, 2H^c, 6H^c, 3H^c and 1H^c, respectively. The chromosomes H1 and H6 were designated as 7H^c and 4H^c, respectively, by referring to SSR markers located on rye chromosomes.
- GISH/FISH,
- Alien chromosome lines,
- Homoeologous group

These authors contribute equally to this article.

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References(49)

References

[1]	Armstrong, K., Fedak, G., Molnar, S. et al.
[2]	Bassam, B.J., Gresshoff, P.M. Silver staining DNA in polyacrylamide gels Nat. Protoc., 2 (2007),pp. 2649-2654
[3]	Bedbrook, J.R., Jones, J., O’Dell, M. et al. Cell, 19 (1980),pp. 545-560
[4]	Chen, P., Qi, L., Zhou, B. et al. Theor. Appl. Genet., 91 (1995),pp. 1125-1128
[5]	Chen, Q., Conner, R.L., Li, H. et al. Genome, 46 (2003),pp. 135-145
[6]	De Bustos, A., Cuadrado, A., Soler, C. et al. Chromosome Res., 4 (1996),pp. 491-499
[7]	Devos, K.M., Atkinson, M.D., Chinoy, C.N. et al. Chromosome rearrangements in the rye genome relative to that of wheat Theor. Appl. Genet., 85 (1993),pp. 673-680
[8]	Devos, K.M., Atkinson, M.D., Chinoy, C.N. et al. RFLP- based genetic map of the homoeologous group 3 chromosomes of wheat and rye Theor. Appl. Genet., 83 (1992),pp. 931-939
[9]	Devos, K.M., Gale, M.D. Comparative genetics in the grasses Plant Mol. Biol., 35 (1997),pp. 3-15
[10]	Gerlach, W.L., Bedbrook, J.R. Cloning and characterization of ribosomal RNA genes from wheat and barley Nucleic Acids Res., 7 (1979),pp. 1869-1885
[11]	Gerlach, W.L., Dyer, T.A. Sequence organization of the repeating units in the nucleus of wheat which contain 5S rDNA genes Nucleic Acids Res., 8 (1980),pp. 4851-4865
[12]	Gill, B.S., Fribe, B., Endo, T.R. Genome, 34 (1991),pp. 830-839
[13]	Gupta, P.K., Fedak, G. J. Hered., 76 (1985),pp. 365-368
[14]	Hackauf, B., Rudd, S., Van der Voort, J.R. et al. Theor. Appl. Genet., 118 (2009),pp. 371-384
[15]	Hackauf, B., Wehling, P. Identification of microsatellite polymorphisms in an expressed portion of the rye genome Plant Breed., 121 (2002),pp. 17-25
[16]	Hackauf, B., Wehling, P. Development of microsatellite markers in rye: map construction Plant Breed. Seed Sci., 48 (2003),pp. 143-151
[17]	International Brachypodium Initiative Nature, 463 (2010),pp. 763-768
[18]	Jia, J., Zhao, S., Kong, X. et al. Nature, 496 (2013),pp. 91-95
[19]	Jiang, J., Friebe, B., Gill, B.S. Recent advances in alien gene transfer in wheat Euphytica, 73 (1994),pp. 199-212
[20]	Koller, O.L., Zeller, F.J. The homoeologous relationships of rye chromosomes 4R and 7R with wheat chromosomes Genetic. Resour., 28 (1976),pp. 177-188
[21]	Kong, F., Wang, H., Cao, A. et al. J. Genet. Genomics, 35 (2008),pp. 673-678
[22]	Kong, F., Wang, H., Zhao, Y. et al. Giemsa C-banding and karyotype analysis of California barley chromosomes Acta Agrestia Sin., 15 (2007),pp. 103-108
[23]	Le, H.T., Armstrong, K.C., Miki, B. Plant Mol. Biol. Rep., 7 (1989),pp. 150-158
[24]	Lelley, T., Kazman, E., Devos, K.M. et al. Use of RFLPs to determine the chromosome composition of tetraploid triticale (A/B)(A/B)RR Genome, 38 (1995),pp. 250-254
[25]	Levan, A., Fredga, K., Sandbreg, A.A. Nomenclature for centromeric position on chromosomes Hereditas, 52 (1964),pp. 201-220
[26]	Li, H., Gill, B.S., Wang, X.-E. et al. Genet. Resour. Crop Evol., 58 (2011),pp. 667-678
[27]	McIntyre, C.L., Pereira, S., Moran, L.B. et al. Genome, 33 (1990),pp. 635-640
[28]	Mirzaghaderi, G., Shahsevand Hassani, H., Karimzadeh, G. Genet. Resour. Crop Evol., 57 (2010),pp. 319-324
[29]	Molnár-Láng, M., Linc, G., Friebe, B. et al. Euphytica, 112 (2000),pp. 117-123
[30]	Nagy, E.D., Molnár-Láng, M., Linc, G. et al. Identification of wheat-barley translocations by sequential GISH and two-colour FISH in combination with the use of genetically mapped barley SSR markers Genome, 45 (2002),pp. 1238-1247
[31]	Naranjo, T., Roca, A., Goicoechea, P.G. et al. Arm homoeology of wheat and rye chromosomes Genome, 29 (1987),pp. 873-882
[32]	Qi, L., Chen, P., Liu, D. et al. Genes Genet. Syst., 74 (1999),pp. 77-82
[33]	Qi, L., Echalier, B., Chao, S. et al. A chromosome bin map of 16,000 expressed sequence tag loci and distribution of genes among the three genomes of polyploid wheat Genetics, 168 (2004),pp. 701-712
[34]	Qi, L., Friebe, B., Zhang, P. et al. Homoeologous recombination, chromosome engineering and crop improvement Chromosome Res., 15 (2007),pp. 3-19
[35]	Qi, Z., Du, P., Qian, B. et al. Theor. Appl. Genet., 121 (2010),pp. 589-597
[36]	Saal, B., Wricke, G. Genome, 42 (1999),pp. 964-972
[37]	Schwarzacher, T., Leitch, A.R., Bennett, M.D. et al. Ann. Bot., 64 (1989),pp. 315-324
[38]	Sears, E.R. Brookhaven Symp. Biol., 9 (1956),pp. 1-22
[39]	Sepsi, A., Molnár, I., Szalay, D. et al. Theor. Appl. Genet., 116 (2008),pp. 825-834
[40]	Sharma, H.C., Gill, B.S. Current status of wide hybridization in wheat Euphytica, 32 (1983),pp. 17-31
[41]	Sherman, J.D., Fenwick, A.L., Namuth, D.M. et al. A barley RFLP map: alignment of three barley maps and comparisons Gramineae species Theor. Appl. Genet., 91 (1995),pp. 681-690
[42]	Somers, D.J., Isaac, P., Edwards, K. Theor. Appl. Genet., 109 (2004),pp. 1105-1114
[43]	Taketa, S., Ando, H., Takeda, K. et al. Theor. Appl. Genet., 100 (2000),pp. 169-176
[44]	Taketa, S., Ando, H., Takeda, K. et al. Ann. Bot., 96 (2005),pp. 23-33
[45]	Taketa, S., Ando, H., Takeda, K. et al. Heredity, 86 (2001),pp. 522-530
[46]	Varshney, R.K., Graner, A., Sorrells, M.E. Genic microsatellite markers in plants: features and applications Trends Biotechnol., 23 (2005),pp. 48-55
[47]	Varshney, R.K., Korzun, V., Börner, A.
[48]	Weng, Y., Lazar, M.D. Comparison of homoeologous group-6 short arm physical maps of wheat and barley reveals a similar distribution of recombinogenic and gene-rich regions Theor. Appl. Genet., 104 (2002),pp. 1078-1085
[49]	Zhou, J., Yang, Z., Li, G. et al. Plant Syst. Evol., 287 (2010),pp. 49-56