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Volume 41 Issue 7
Jul.  2014
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Article Navigation >  Journal of Genetics and Genomics  > 2014  >  41(7): 379-396

Transcriptomic Response to Yersinia pestis: RIG-I Like Receptor Signaling Response Is Detrimental to the Host against Plague

doi: 10.1016/j.jgg.2014.05.006
  • a 

    State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Beijing 100071, China

  • b 

    Qinghai Institute for Endemic Disease Prevention and Control of Qinghai Province, Xining 811602, China

  • c 

    BGI-Shenzhen, Shenzhen 518083, China

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  • Corresponding author: E-mail address: zongmindu@gmail.com (Zongmin Du); E-mail address: ruifuyang@gmail.com (Ruifu Yang)
  • Received Date: 2014-04-24
  • Accepted Date: 2014-05-14
  • Rev Recd Date: 2014-05-09
    • Available Online: 2014-06-02
  • Publish Date: 2014-07-20
    Abstract
  • Bacterial pathogens have evolved various mechanisms to modulate host immune responses for successful infection. In this study, RNA-sequencing technology was used to analyze the responses of human monocytes THP1 to Yersinia pestis infection. Over 6000 genes were differentially expressed over the 12 h infection. Kinetic responses of pathogen recognition receptor signaling pathways, apoptosis, antigen processing, and presentation pathway and coagulation system were analyzed in detail. Among them, RIG-I-like receptor (RLR) signaling pathway, which was established for antiviral defense, was significantly affected. Mice lacking MAVS, the adaptor of the RLR signaling pathway, were less sensitive to infection and exhibited lower IFN-β production, higher Th1-type cytokines IFN-γ and IL-12 production, and lower Th2-type cytokines IL-4 and IL-13 production in the serum compared with wild-type mice. Moreover, infection of pathogenic bacteria other than Y. pestis also altered the expression of the RLR pathway, suggesting that the response of RLR pathway to bacterial infection is a universal mechanism.
    • These authors contribute equally to this work.
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