Nodal Promotes mir206 Expression to Control Convergence and Extension Movements During Zebrafish Gastrulation

Xiuli Liu; Yuanqing Ma; Congwei Zhang; Shi Wei; Yu Cao; Qiang Wang

doi:10.1016/j.jgg.2013.07.001

Volume 40 Issue 10

Oct. 2013

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Nodal Promotes mir206 Expression to Control Convergence and Extension Movements During Zebrafish Gastrulation

doi: 10.1016/j.jgg.2013.07.001

a
State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China
b
Department of Outpatient Surgery, Anguo City Hospital of Hebei Province, Baoding 071200, China

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Corresponding author: E-mail address: qiangwang@ioz.ac.cn (Qiang Wang)
Received Date: 2013-03-06
Accepted Date: 2013-07-22
Rev Recd Date: 2013-07-15

Available Online: 2013-08-08

Publish Date: 2013-10-20

Abstract

Abstract

Nodal, a member of the transforming growth factor β (TGF-β) superfamily, has been shown to play a role in mesendoderm induction and gastrulation movements. The activity of Nodal signaling can be modulated by microRNAs (miRNAs) as previously reported, but little is known about which miRNAs are regulated by Nodal during gastrulation. In the present study, we found that the expression of mir206, one of the most abundant miRNAs during zebrafish early embryo development, is regulated by Nodal signaling. Abrogation of Nodal signal activity results in defective convergence and extension (CE) movements, and these cell migration defects can be rescued by supplying an excess ofmir206, suggesting that mir206 acts downstream of Nodal signaling to regulate CE movements. Furthermore, in mir206 morphants, the expression of cell adhesion molecule E-cadherin is significantly increased, while the key transcriptional repressor of E-cadherin, snail1a, is depressed. Our study uncovers a novel mechanism by which Nodal-regulated mir206 modulates gastrulation movements in connection with the Snail/E-cadherin pathway.
- Nodal,
- Convergence and extension,
- E-cadherin

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References(38)

References

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