An in vivo Transient Expression System Can Be Applied for Rapid and Effective Selection of Artificial MicroRNA Constructs for Plant Stable Genetic Transformation

Basdeo Bhagwat; Ming Chi; Li Su; Haifeng Tang; Guiliang Tang; Yu Xiang

doi:10.1016/j.jgg.2013.03.012

Volume 40 Issue 5

May 2013

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Article Navigation > Journal of Genetics and Genomics > 2013 > 40(5): 261-270

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An in vivo Transient Expression System Can Be Applied for Rapid and Effective Selection of Artificial MicroRNA Constructs for Plant Stable Genetic Transformation

doi: 10.1016/j.jgg.2013.03.012

Basdeo Bhagwat^a,
Ming Chi^{a, b},
Li Su^{a, c},
Haifeng Tang^{d, e},
Guiliang Tang^{d, e, f},
Yu Xiang^a

a
Agriculture and Agri-Food Canada, Pacific Agri-Food Research Center, Summerland, British Columbia V0H 1Z0, Canada
b
College of Forestry, Northwest A & F University, Yangling 712100, China
c
College of Horticulture, Northwest A & F University, Yangling 712100, China
d
Key Laboratory of Physiology, Ecology, and Genetic Improvement of Food Crop in Henan Province, College of Agriculture, Henan Agricultural University, Zhengzhou 450002, China
e
Department of Plant and Soil Sciences and KTRDC, University of Kentucky, Lexington, KY 40546, USA
f
Department of Biological Sciences, Michigan Technological University, Houghton, MI 49931, USA

More Information

Corresponding author: E-mail address: gtang1@mtu.edu (Guiliang Tang); E-mail address: yu.xiang@agr.gc.ca (Yu Xiang)
Received Date: 2013-03-06
Accepted Date: 2013-03-24
Rev Recd Date: 2013-03-23

Available Online: 2013-04-11

Publish Date: 2013-05-20

Abstract

Abstract

The utility of artificial microRNAs (amiRNAs) to induce loss of gene function has been reported for many plant species, but expression efficiency of the different amiRNA constructs in different transgenic plants was less predictable. In this study, expressions of amiRNAs through the gene backbone of Arabidopsis miR168a were examined by both Agrobacterium-mediated transient expression and stable plant genetic transformation. A corresponding trend in expression of amiRNAs by the same amiRNA constructs between the transient and the stable expression systems was observed in the experiments. Plant genetic transformation of the constructs that were highly expressible in amiRNAs in the transient agro-infiltration assays resulted in generation of transgenic lines with high level of amiRNAs. This provides a simple method for rapid and effective selection of amiRNA constructs used for a time-consuming genetic transformation in plants.
- Artificial microRNA,
- Plant genetic transformation

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References(38)

References

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