Identifying MicroRNA and mRNA Expression Profiles in Embryonic Stem Cells Derived from Parthenogenetic, Androgenetic and Fertilized Blastocysts

Xiang-Shun Cui; Xing-Hui Shen; Shao-Chen Sun; Sun-Wha Cho; Young-Tae Heo; Yong-Kook Kang; Teruhiko Wakayama; Nam-Hyung Kim

doi:10.1016/j.jgg.2013.03.006

Volume 40 Issue 4

Apr. 2013

Turn off MathJax

Article Contents

Article Navigation > Journal of Genetics and Genomics > 2013 > 40(4): 189-200

PDF( 1082 KB)

Identifying MicroRNA and mRNA Expression Profiles in Embryonic Stem Cells Derived from Parthenogenetic, Androgenetic and Fertilized Blastocysts

doi: 10.1016/j.jgg.2013.03.006

a
Department of Animal Science, Chungbuk National University, Gaeshin-dong, Cheongju, Chungbuk 361-763, South Korea
b
Department of Histology and Embryology, Harbin Medical University, Harbin 150081, China
c
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China
d
Laboratory of Development and Differentiation, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806, South Korea
e
Center for Developmental Biology, RIKEN Kobe, Kobe 650-0047, Japan

More Information

Corresponding author: E-mail address: nhkim@chungbuk.ac.kr (Nam-Hyung Kim)
Received Date: 2012-12-31
Accepted Date: 2013-03-14
Rev Recd Date: 2013-03-13

Available Online: 2013-03-21

Publish Date: 2013-04-20

Abstract

Abstract

MicroRNAs (miRNAs) are a class of highly conserved small non-coding RNA molecules that play a pivotal role in several cellular functions. In this study, miRNA and messenger RNA (mRNA) profiles were examined by Illumina microarray in mouse embryonic stem cells (ESCs) derived from parthenogenetic, androgenetic, and fertilized blastocysts. The global analysis of miRNA-mRNA target pairs provided insight into the role of miRNAs in gene expression. Results showed that a total of 125 miRNAs and 2394 mRNAs were differentially expressed between androgenetic ESCs (aESCs) and fertilized ESCs (fESCs), a total of 42 miRNAs and 87 mRNAs were differentially expressed between parthenogenetic ESCs (pESCs) and fESCs, and a total of 99 miRNAs and 1788 mRNAs were differentially expressed between aESCs and pESCs. In addition, a total of 575, 5 and 376 miRNA-mRNA target pairs were observed in aESCs vs. fESCs, pESCs vs. fESCs, and aESCs vs. pESCs, respectively. Furthermore, 15 known imprinted genes and 16 putative uniparentally expressed miRNAs with high expression levels were confirmed by both microarray and real-time RT-PCR. Finally, transfection of miRNA inhibitors was performed to validate the regulatory relationship between putative maternally expressed miRNAs and target mRNAs. Inhibition of miR-880 increased the expression ofPeg3, Dyrk1b, and Prrg2 mRNA, inhibition of miR-363 increased the expression of Nfat5 and Soat1 mRNA, and inhibition of miR-883b-5p increased Nfat5, Tacstd2, and Ppapdc1 mRNA. These results warrant a functional study to fully understand the underlying regulation of genomic imprinting in early embryo development.
- Embryonic stem cell,
- Parthenogenetic,
- Androgenetic,
- Fertilized,
- Microarray,
- miRNA-mRNA network

FullText(HTML)

References(28)

References

[1]	Bartel, D.P. MicroRNAs: genomics, biogenesis, mechanism, and function Cell, 116 (2004),pp. 281-297
[2]	Barton, S.C., Surani, M.A., Norris, M.L. Role of paternal and maternal genomes in mouse development Nature, 311 (1984),pp. 374-376
[3]	Cui, X., Shen, X., Lee, C. et al. Analysis of proteomic profiling of mouse embryonic stem cells derived from fertilized, parthenogenetic and androgenetic blastocysts Stem Cell Discov., 1 (2011),pp. 1-15
[4]	Cui, X.S., Li, X.Y., Jeong, Y.J. et al. Gene expression of cox5a, 5b, or 6b1 and their roles in preimplantation mouse embryos Biol. Reprod., 74 (2006),pp. 601-610
[5]	Cui, X.S., Li, X.Y., Kim, N.H. Biochem. Biophys. Res. Commun., 352 (2007),pp. 709-715
[6]	Cui, X.S., Zhang, D.X., Ko, Y.G. et al. Aberrant epigenetic reprogramming of imprinted microRNA-127 and Rtl1 in cloned mouse embryos Biochem. Biophys. Res. Commun., 379 (2009),pp. 390-394
[7]	Dighe, V., Clepper, L., Pedersen, D. et al. Heterozygous embryonic stem cell lines derived from nonhuman primate parthenotes Stem Cells, 26 (2008),pp. 756-766
[8]	Filipowicz, W., Bhattacharyya, S.N., Sonenberg, N. Mechanisms of post-transcriptional regulation by microRNAs: are the answers in sight? Nat. Rev. Genet., 9 (2008),pp. 102-114
[9]	Gardiner, E., Beveridge, N.J., Wu, J.Q. et al. Imprinted DLK1-DIO3 region of 14q32 defines a schizophrenia-associated miRNA signature in peripheral blood mononuclear cells Mol. Psychiatry, 17 (2012),pp. 827-840
[10]	Grimson, A., Farh, K.K., Johnston, W.K. et al. MicroRNA targeting specificity in mammals: determinants beyond seed pairing Mol. Cell, 27 (2007),pp. 91-105
[11]	Hikichi, T., Ohta, H., Wakayama, S. et al. Functional full-term placentas formed from parthenogenetic embryos using serial nuclear transfer Development, 137 (2010),pp. 2841-2847
[12]	Hikichi, T., Wakayama, S., Mizutani, E. et al. Differentiation potential of parthenogenetic embryonic stem cells is improved by nuclear transfer Stem Cells, 25 (2007),pp. 46-53
[13]	Kaufman, M.H., Barton, S.C., Surani, M.A. Normal postimplantation development of mouse parthenogenetic embryos to the forelimb bud stage Nature, 265 (1977),pp. 53-55
[14]	Kim, K., Lerou, P., Yabuuchi, A. et al. Histocompatible embryonic stem cells by parthenogenesis Science, 315 (2007),pp. 482-486
[15]	Kono, T. Genomic imprinting is a barrier to parthenogenesis in mammals Cytogenet. Genome Res., 113 (2006),pp. 31-35
[16]	Lei, L., Jin, S., Gonzalez, G. et al. The regulatory role of Dicer in folliculogenesis in mice Mol. Cell. Endocrinol., 315 (2010),pp. 63-73
[17]	Lewis, B.P., Burge, C.B., Bartel, D.P. Conserved seed pairing, often flanked by adenosines, indicates that thousands of human genes are microRNA targets Cell, 120 (2005),pp. 15-20
[18]	Livak, K.J., Schmittgen, T.D. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method Methods, 25 (2001),pp. 402-408
[19]	Mattick, J.S., Makunin, I.V. Small regulatory RNAs in mammals Hum. Mol. Genet., 14 (2005),pp. R121-R132
[20]	Narasimha, M., Barton, S.C., Surani, M.A. The role of the paternal genome in the development of the mouse germ line Curr. Biol., 7 (1997),pp. 881-884
[21]	Nomura, T., Kimura, M., Horii, T. et al. MeCP2-dependent repression of an imprinted miR-184 released by depolarization Hum. Mol. Genet., 17 (2008),pp. 1192-1199
[22]	Peters, J., Robson, J.E. Imprinted noncoding RNAs Mamm. Genome, 19 (2008),pp. 493-502
[23]	Royo, H., Cavaille, J. Non-coding RNAs in imprinted gene clusters Biol. Cell, 100 (2008),pp. 149-166
[24]	Seitz, H., Royo, H., Bortolin, M.L. et al. A large imprinted microRNA gene cluster at the mouse Dlk1-Gtl2 domain Genome Res., 14 (2004),pp. 1741-1748
[25]	Seitz, H., Youngson, N., Lin, S.P. et al. Imprinted microRNA genes transcribed antisense to a reciprocally imprinted retrotransposon-like gene Nat. Genet., 34 (2003),pp. 261-262
[26]	Steel, R.G.D., Torrie, J.H.
[27]	Wakayama, S., Ohta, H., Kishigami, S. et al. Establishment of male and female nuclear transfer embryonic stem cell lines from different mouse strains and tissues Biol. Reprod., 72 (2005),pp. 932-936
[28]	Zhang, R., Peng, Y., Wang, W. et al. Rapid evolution of an X-linked microRNA cluster in primates Genome Res., 17 (2007),pp. 612-617