Large-Scale Forward Genetic Screening Analysis of Development of Hematopoiesis in Zebrafish

Kun Wang; Zhibin Huang; Lingfeng Zhao; Wei Liu; Xiaohui Chen; Ping Meng; Qing Lin; Yali Chi; Mengchang Xu; Ning Ma; Yiyue Zhang; Wenqing Zhang

doi:10.1016/j.jgg.2012.07.008

Volume 39 Issue 9

Sep. 2012

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Article Navigation > Journal of Genetics and Genomics > 2012 > 39(9): 473-480

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Large-Scale Forward Genetic Screening Analysis of Development of Hematopoiesis in Zebrafish

doi: 10.1016/j.jgg.2012.07.008

Key Laboratory of Zebrafish Modeling and Drug Screening for Human Diseases of Guangdong Higher Education Institutes, Department of Cell Biology, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, China

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Corresponding author: E-mail address: yiyue@smu.edu.cn (Yiyue Zhang); E-mail address: zwq.smu@gmail.com (Wenqing Zhang)
Received Date: 2012-04-27
Accepted Date: 2012-07-25
Rev Recd Date: 2012-07-24

Available Online: 2012-08-11

Publish Date: 2012-09-20

Abstract

Abstract

Zebrafish is a powerful model for the investigation of hematopoiesis. In order to isolate novel mutants with hematopoietic defects, large-scale mutagenesis screening of zebrafish was performed. By scoring specific hematopoietic markers, 52 mutants were identified and then classified into four types based on specific phenotypic traits. Each mutant represented a putative mutation of a gene regulating the relevant aspect of hematopoiesis, including early macrophage development, early granulopoiesis, embryonic myelopoiesis, and definitive erythropoiesis/lymphopoiesis. Our method should be applicable for other types of genetic screening in zebrafish. In addition, further study of the mutants we identified may help to unveil the molecular basis of hematopoiesis.
- Zebrafish,
- Large-scale screening,
- Hematopoiesis

These authors contributed equally to this work.

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References(29)

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