Ribosome Biogenesis Factor Bms1-like Is Essential for Liver Development in Zebrafish

Yong Wang; Yue Luo; Yunhan Hong; Jinrong Peng; Lijan Lo

doi:10.1016/j.jgg.2012.07.007

Volume 39 Issue 9

Sep. 2012

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Article Navigation > Journal of Genetics and Genomics > 2012 > 39(9): 451-462

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Ribosome Biogenesis Factor Bms1-like Is Essential for Liver Development in Zebrafish

doi: 10.1016/j.jgg.2012.07.007

a
College of Animal Sciences, Zhejiang University, Hangzhou 310058, China
b
Department of Biological Sciences, National University of Singapore, Science Drive 4, Singapore 117543, Singapore

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Corresponding author: E-mail address: dbshyh@nus.edu.sg (Yunhan Hong); E-mail address: pengjr@zju.edu.cn (Jinrong Peng); E-mail address: g0403022@zju.edu.cn (Lijan Lo)
Received Date: 2012-05-06
Accepted Date: 2012-07-12
Rev Recd Date: 2012-07-11

Available Online: 2012-08-11

Publish Date: 2012-09-20

Abstract

Abstract

Ribosome biogenesis in the nucleolus requires numerous nucleolar proteins and small non-coding RNAs. Among them is ribosome biogenesis factor Bms1, which is highly conserved from yeast to human. In yeast, Bms1 initiates ribosome biogenesis through recruiting Rcl1 to pre-ribosomes. However, little is known about the biological function of Bms1 in vertebrates. Here we report that Bms1 plays an essential role in zebrafish liver development. We identified a zebrafishbms1l mutant which carries a T to A mutation in the gene bms1-like (bms1l). This mutation results in L¹⁵² to Q¹⁵² substitution in a GTPase motif in Bms1l. Surprisingly, bms1l mutation confers hypoplasia specifically in the liver, exocrine pancreas and intestine after 3 days post-fertilization (dpf). Consistent with the bms1l mutant phenotypes, whole-mount in situ hybridization (WISH) on wild type embryos showed that bms1l transcripts are abundant in the entire digestive tract and its accessory organs. Immunostaining for phospho-Histone 3 (P-H3) and TUNEL assay revealed that impairment of hepatoblast proliferation rather than cell apoptosis is one of the consequences of bms1l giving rise to an under-developed liver. Therefore, our findings demonstrate that Bms1l is necessary for zebrafish liver development.
- Liver development,
- Digestive organ development,
- Ribosome biogenesis,
- Bms1-like,
- Zebrafish

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References(37)

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