A Pyramid Breeding of Eight Grain-yield Related Quantitative Trait Loci Based on Marker-assistant and Phenotype Selection in Rice (Oryza sativa L.)

Guo Zong; Ahong Wang; Lu Wang; Guohua Liang; Minghong Gu; Tao Sang; Bin Han

doi:10.1016/j.jgg.2012.06.004

Volume 39 Issue 7

Jul. 2012

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Article Navigation > Journal of Genetics and Genomics > 2012 > 39(7): 335-350

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A Pyramid Breeding of Eight Grain-yield Related Quantitative Trait Loci Based on Marker-assistant and Phenotype Selection in Rice (Oryza sativa L.)

doi: 10.1016/j.jgg.2012.06.004

a
National Center for Gene Research and Institute of Plant Physiology and Ecology, Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences, Shanghai 200233, China
b
Jiangsu Key Laboratory of Crop Genetics and Physiology/Key Laboratory of the Ministry of Education for Plant Functional Genomics, Yangzhou University, 88 Daxue Road, Yangzhou 225009, China
c
State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China

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Corresponding author: E-mail address: bhan@ncgr.ac.cn (Bin Han)
Received Date: 2012-04-18
Accepted Date: 2012-06-18
Rev Recd Date: 2012-05-17

Available Online: 2012-06-29

Publish Date: 2012-07-20

Abstract

Abstract

1000-Grain weight and spikelet number per panicle are two important components for rice grain yield. In our previous study, eight quantitative trait loci (QTLs) conferring spikelet number per panicle and 1000-grain weight were mapped through sequencing-based genotyping of 150 rice recombinant inbred lines (RILs). In this study, we validated the effects of four QTLs from Nipponbare using chromosome segment substitution lines (CSSLs), and pyramided eight grain yield related QTLs. The new lines containing the eight QTLs with positive effects showed increased panicle and spikelet size as compared with the parent variety 93-11. We further proposed a novel pyramid breeding scheme based on marker-assistant and phenotype selection (MAPS). This scheme allowed pyramiding of as many as 24 QTLs at a single hybridization without massive cross work. This study provided insights into the molecular basis of rice grain yield for direct wealth for high-yielding rice breeding.
- Rice,
- Spikelet number,
- 1000-Grain weight,
- Quantitative trait loci,
- Pyramid breeding,
- Marker assisted and phenotype selection

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References(39)

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