A Systematic Phenotypic Screen of F-box Genes Through a Tissue-specific RNAi-based Approach in Drosophila

Wen Dui; Wei Lu; Jun Ma; Renjie Jiao

doi:10.1016/j.jgg.2012.05.009

Volume 39 Issue 8

Aug. 2012

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Article Navigation > Journal of Genetics and Genomics > 2012 > 39(8): 397-413

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A Systematic Phenotypic Screen of F-box Genes Through a Tissue-specific RNAi-based Approach in Drosophila

doi: 10.1016/j.jgg.2012.05.009

Wen Dui^{a, b, c},
Wei Lu^a,
Jun Ma^{c, d},
Renjie Jiao^a

a
State Key Laboratory of Brain and Cognitive Science, Institute of Biophysics, Chinese Academy of Sciences, Datun Road 15, Beijing 100101, China
b
Graduate School of Chinese Academy of Sciences, Beijing 100080, China
c
Division of Biomedical Informatics, Cincinnati Children's Research Foundation, 3333 Burnet Avenue, Cincinnati, OH 45229, USA
d
Division of Developmental Biology, Cincinnati Children's Research Foundation, 3333 Burnet Avenue, Cincinnati, OH 45229, USA

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Corresponding author: E-mail address: jun.ma@cchmc.org (Jun Ma); E-mail address: rjiao@sun5.ibp.ac.cn (Renjie Jiao)
Received Date: 2012-04-23
Accepted Date: 2012-05-30
Rev Recd Date: 2012-05-25

Available Online: 2012-07-14

Publish Date: 2012-08-20

Abstract

Abstract

F-box proteins are components of the SCF (SkpA-Cullin 1-F-box) E3 ligase complexes, acting as the specificity-determinants in targeting substrate proteins for ubiquitination and degradation. In humans, at least 22 out of 75 F-box proteins have experimentally documented substrates, whereas in Drosophila 12 F-box proteins have been characterized with known substrates. To systematically investigate the genetic and molecular functions of F-box proteins in Drosophila, we performed a survey of the literature and databases. We identified 45 Drosophila genes that encode proteins containing at least one F-box domain. We collected publically available RNAi lines against these genes and used them in a tissue-specific RNAi-based phenotypic screen. Here, we present our systematic phenotypic dataset from the eye, the wing and the notum. This dataset is the first of its kind and represents a useful resource for future studies of the molecular and genetic functions of F-box genes in Drosophila. Our results show that, as expected, F-box genes in Drosophila have regulatory roles in a diverse array of processes including cell proliferation, cell growth, signal transduction, and cellular and animal survival.
- Tissue-specific,
- RNAi-based,
- Phenotypic screen

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References(86)

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